uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Proteome analysis of bronchoalveolar lavage from calves infected with bovine respiratory syncytial virus-Insights in pathogenesis and perspectives for new treatments
Swedish Univ Agr Sci, Host Pathogen Interact Grp, Dept Clin Sci, Uppsala, Sweden..
Swedish Univ Agr Sci, Host Pathogen Interact Grp, Dept Clin Sci, Uppsala, Sweden..
Swedish Univ Agr Sci, Host Pathogen Interact Grp, Dept Clin Sci, Uppsala, Sweden..
Swedish Univ Agr Sci, Sect Pathol, Dept Biomed Sci & Vet Publ Hlth, Uppsala, Sweden..
Show others and affiliations
2017 (English)In: PLoS Pathogens, ISSN 1553-7366, E-ISSN 1553-7374, Vol. 12, no 10, article id e0186594Article in journal (Refereed) Published
Abstract [en]

Human and bovine respiratory syncytial viruses (HRSV/BRSV) are major causes of severe lower respiratory tract infections in children and calves, respectively. Shared epidemiological, clinical, pathological and genetic characteristics of these viruses make comparative research highly relevant. To characterise the host response against BRSV infection, bronchoalveolar lavage supernatant (BAL) from i) non-vaccinated, BRSV-infected ii) vaccinated, BRSV-infected and iii) non-infected calves was analysed by tandem mass spectrometry. Proteins were semi-quantified and protein expression was validated by immunoblotting. Correlations between selected proteins and pathology, clinical signs and virus shedding were investigated. Calves with BRSV-induced disease had increased total protein concentrations and a decreased number of proteins identified in BAL. The protein profile was characterised by neutrophil activation and a reduction in identified antioxidant enzymes. The presence of neutrophils in alveolar septa, the expression level of neutrophil-related or antioxidant proteins and LZTFL1 correlated significantly with disease. Citrullinated histone 3, an indicator of extracellular traps (ETs), was only detected in non-vaccinated, BRSV-infected animals. By bringing disequilibrium in the release and detoxification of reactive oxygen species, generating ETs and causing elastine degradation, exaggerated neutrophil responses might exacerbate RSV-induced disease. Neutrophil-mitigating or antioxidant treatments should be further explored.

Place, publisher, year, edition, pages
PUBLIC LIBRARY SCIENCE , 2017. Vol. 12, no 10, article id e0186594
National Category
Agricultural and Veterinary sciences
Identifiers
URN: urn:nbn:se:uu:diva-337190DOI: 10.1371/journal.pone.0186594ISI: 000413054800048PubMedID: 29036182OAI: oai:DiVA.org:uu-337190DiVA, id: diva2:1168612
Available from: 2017-12-21 Created: 2017-12-21 Last updated: 2018-01-31

Open Access in DiVA

fulltext(12076 kB)2 downloads
File information
File name FULLTEXT01.pdfFile size 12076 kBChecksum SHA-512
719c172249f284242ca5b5242cdc252cac74c4e8eb914f5262f0d13cee1677424db78b45e855b72ed01976f6de6315379bf0d360ce226e59d6f183772ea1d657
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Bergström, Sara K.

Search in DiVA

By author/editor
Bergström, Sara K.
By organisation
Analytical ChemistryScience for Life Laboratory, SciLifeLab
In the same journal
PLoS Pathogens
Agricultural and Veterinary sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 2 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 22 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf