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Temporal characterization of the non-structural Adenovirus type 2 proteome and phosphoproteome using high-resolving mass spectrometry
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. (Bergquist/Lind)
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. (Lind)
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. Beijer Lab, Rudbeck Lab. (Pettersson)
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. (Lind)
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2017 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 511, p. 240-248Article in journal (Refereed) Published
Abstract [en]

The proteome and phosphoproteome of non-structural proteins of Adenovirus type 2 (Ad2) were time resolved using a developed mass spectrometry approach. These proteins are expressed by the viral genome and important for the infection process, but not part of the virus particle. We unambiguously confirm the existence of 95% of the viral proteins predicted to be encoded by the viral genome. Most non-structural proteins peaked in expression at late time post infection. We identified 27 non-redundant sites of phosphorylation on seven different non-structural proteins. The most heavily phosphorylated protein was the DNA binding protein (DBP) with 15 different sites. The phosphorylation occupancy rate could be calculated and monitored with time post infection for 15 phosphorylated sites on various proteins. In the DBP, phosphorylations with time-dependent relation were observed. The findings show the complexity of the Ad2 non-structural proteins and opens up a discussion for potential new drug targets.

Place, publisher, year, edition, pages
Uppsala: ACADEMIC PRESS INC ELSEVIER SCIENCE , 2017. Vol. 511, p. 240-248
Keywords [en]
Adenovirus type 2, Non-structural proteins, Time series, Protein expression, Phosphorylation, Mass spectrometry
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-337189DOI: 10.1016/j.virol.2017.08.032ISI: 000412791500027PubMedID: 28915437OAI: oai:DiVA.org:uu-337189DiVA, id: diva2:1168615
Funder
Åke Wiberg Foundation, M14-0127Magnus Bergvall Foundation, 2015-01200, 2016-01675Carl Tryggers foundation , CST 15:57Available from: 2017-12-21 Created: 2017-12-21 Last updated: 2018-01-02Bibliographically approved

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Källsten, MalinZhao, HongxingValdés, AlbertoPettersson, Ulf

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Källsten, MalinZhao, HongxingValdés, AlbertoPettersson, UlfLind, Sara Bergstrom
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Analytical ChemistryScience for Life Laboratory, SciLifeLabDepartment of Immunology, Genetics and PathologyMedicinsk genetik och genomik
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