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H1N1 vaccination in Sjogren's syndrome triggers polyclonal B cell activation and promotes autoantibody production
Karolinska Univ Hosptial, Karolinska Inst, Dept Med, Stockholm, Sweden..
Karolinska Univ Hosptial, Karolinska Inst, Dept Med, Stockholm, Sweden..
Karolinska Univ Hosptial, Karolinska Inst, Dept Med, Stockholm, Sweden..
Karolinska Univ Hosptial, Karolinska Inst, Dept Med, Stockholm, Sweden..
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2017 (English)In: Annals of the Rheumatic Diseases, ISSN 0003-4967, E-ISSN 1468-2060, Vol. 76, no 10, p. 1755-1763Article in journal (Refereed) Published
Abstract [en]

Objectives

Vaccination of patients with rheumatic disease has been reported to result in lower antibody titres than in healthy individuals. However, studies primarily include patients on immunosuppressive therapy. Here, we investigated the immune response of treatment-naive patients diagnosed with primary Sjogren's syndrome (pSS) to an H1N1 influenza vaccine.

Methods

Patients with Sjogren's syndrome without immunomodulatory treatment and age-matched and gender-matched healthy controls were immunised with an H1N1 influenza vaccine and monitored for serological and cellular immune responses. Clinical symptoms were monitored with a standardised form. IgG class switch and plasma cell differentiation were induced in vitro in purified naive B cells of untreated and hydroxychloroquine-treated patients and healthy controls. Gene expression was assessed by NanoString technology.

Results

Surprisingly, treatment-naive patients with Sjogren's syndrome developed higher H1N1 IgG titres of greater avidity than healthy controls on vaccination. Notably, off-target B cells were also triggered resulting in increased anti-EBV and autoantibody titres. Endosomal toll-like receptor activation of naive B cells in vitro revealed a greater propensity of patient-derived cells to differentiate into plasmablasts and higher production of class switched IgG. The amplified plasma cell differentiation and class switch could be induced in cells from healthy donors by preincubation with type 1 interferon, but was abolished in hydroxychloroquine-treated patients and after in vitro exposure of naive B cells to chloroquine.

Conclusions

This comprehensive analysis of the immune response in autoimmune patients to exogenous stimulation identifies a mechanistic basis for the B cell hyperactivity in Sjogren's syndrome, and suggests that caution is warranted when considering vaccination in non-treated autoimmune patients.

Place, publisher, year, edition, pages
2017. Vol. 76, no 10, p. 1755-1763
National Category
Rheumatology and Autoimmunity
Identifiers
URN: urn:nbn:se:uu:diva-336305DOI: 10.1136/annrheumdis-2016-210509ISI: 000410939600028PubMedID: 28760805OAI: oai:DiVA.org:uu-336305DiVA, id: diva2:1176022
Available from: 2018-01-19 Created: 2018-01-19 Last updated: 2018-01-19Bibliographically approved

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