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Fluorinated Substances Cytotoxicity and Influence on Proliferation of Human Breast Basal Epithelial Cells (MCF-10A)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
2018 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Introduction: Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are man-made compounds that are water-, fat- and dirt repellent. Long-chain PFAS have been dominant on the market, until studies showed evidence of increased risk of cancer, developmental toxicity and liver toxicity. Regulations forced industries into change, which resulted in a transition to short-chain PFAS. However, growing evidence points towards similar adverse effects for several short-chained PFAS. This study investigates the toxicity of three short-chain PFAS.

Materials and methods: Human breast epithelial cells (MCF-10A) were exposed to three short-chain PFAS in a varying range of concentrations and time of exposure. Results of cytotoxicity and proliferation were obtained by performing the MTT assay and receptor antagonists were used to investigate the role of each receptor. Also, oxidative stress was evaluated by DCFDA assay.

Results: The investigated compounds demonstrated cytotoxicity at high concentrations (1 mM). Two compounds also induced cytotoxicity at lower concentrations In addition, one of the compounds induced cell proliferation at 500 µM after 72 h exposure.

Conclusion: This study gives a first indication of the investigated compounds induced toxicological effects on human breast epithelial cells. The results contribute to the ongoing research with the aim to investigate the toxicological profiles of new PFAS compounds. 

Place, publisher, year, edition, pages
2018. , p. 23
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:uu:diva-339546OAI: oai:DiVA.org:uu-339546DiVA, id: diva2:1176073
Subject / course
Pharmaceutical Biosciences
Educational program
Master of Science Programme in Pharmacy
Supervisors
Examiners
Available from: 2018-01-23 Created: 2018-01-19 Last updated: 2018-01-23Bibliographically approved

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