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A Pacifastacus leniusculus serine protease interacts with WSSV
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology.
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2017 (English)In: Fish and Shellfish Immunology, ISSN 1050-4648, E-ISSN 1095-9947, Vol. 68, p. 211-219Article in journal (Refereed) Published
Abstract [en]

Serine proteases are involved in many critical physiological processes including virus spread and replication. In the present study, we identified a new clip-domain serine protease (PIcSP) in the crayfish Pacifastacus leniusculus hemocytes, which can interact with the White Spot Syndrome Virus (WSSV) envelope protein VP28. It was characterized by a classic clip domain with six strictly conserved Cys residues, and contained the conserved His-Asp-Ser (H-D-S)motif in the catalytic domain. Furthermore, signal peptide prediction revealed that it has a 16-residue secretion signal peptide. Tissue distribution showed that it was mainly located in P. leniusculus hemocytes, and its expression was increased in hemocytes upon WSSV challenge. In vitro knock down of PIcSP decreased both the expression of VP28 and the WSSV copy number in hematopoietic stem (HPT) cells. Accordingly, these data suggest that the new serine protease may be of importance for WSSV infection into hematopoietic cells.

Place, publisher, year, edition, pages
2017. Vol. 68, p. 211-219
Keywords [en]
Hematopoietic tissue, Invertebrate, Serine protease, Virus, WSSV
National Category
Immunology
Identifiers
URN: urn:nbn:se:uu:diva-335858DOI: 10.1016/j.fsi.2017.07.026ISI: 000411299500022PubMedID: 28705723OAI: oai:DiVA.org:uu-335858DiVA, id: diva2:1177133
Funder
Swedish Research Council, 621-2012-2418Available from: 2018-01-24 Created: 2018-01-24 Last updated: 2018-06-26Bibliographically approved
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Korkut, Gül GizemSöderhäll, IreneSöderhäll, Kenneth

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