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Heparinization of Beta Tricalcium Phosphate: Osteo-immunomodulatory Effects
Univ Politecn Cataluna, Dept Mat Sci & Met Engn, Biomat Biomech & Tissue Engn Grp, EEBE, Ave Eduard Maristany 10-14, Barcelona 08019, Spain; Univ Politecn Cataluna, Barcelona Res Ctr Multiscale Sci & Engn, EEBE, Ave Eduard Maristany 10-14, Barcelona 08019, Spain.
Univ Politecn Cataluna, Dept Mat Sci & Met Engn, Biomat Biomech & Tissue Engn Grp, EEBE, Ave Eduard Maristany 10-14, Barcelona 08019, Spain Univ Politecn Cataluna, Barcelona Res Ctr Multiscale Sci & Engn, EEBE, Ave Eduard Maristany 10-14, Barcelona 08019, Spain.
Univ Politecn Cataluna, Dept Mat Sci & Met Engn, Biomat Biomech & Tissue Engn Grp, EEBE, Ave Eduard Maristany 10-14, Barcelona 08019, Spain Univ Politecn Cataluna, Barcelona Res Ctr Multiscale Sci & Engn, EEBE, Ave Eduard Maristany 10-14, Barcelona 08019, Spain.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences. (Materials in Medicine)
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2018 (English)In: Advanced Healthcare Materials, ISSN 2192-2640, E-ISSN 2192-2659, Vol. 7, no 5, article id 1700867Article in journal (Refereed) Published
Abstract [en]

Immune cells play a vital role in regulating bone dynamics. This has boosted the interest in developing biomaterials that can modulate both the immune and skeletal systems. In this study, calcium phosphates discs (i.e., beta-tricalcium phosphate, β-TCP) are functionalized with heparin to investigate the effects on immune and stem cell responses. The results show that the functionalized surfaces downregulate the release of hydrogen peroxide and proinflammatory cytokines (tumor necrosis factor alpha and interleukin 1 beta) from human monocytes and neutrophils, compared to nonfunctionalized discs. The macrophages show both elongated and round shapes on the two ceramic substrates, but the morphology of cells on heparinized β-TCP tends toward a higher elongation after 72 h. The heparinized substrates support rat mesenchymal stem cell (MSC) adhesion and proliferation, and anticipate the differentiation toward the osteoblastic lineage as compared to β-TCP and control. The coupling between the inflammatory response and osteogenesis is assessed by culturing MSCs with the macrophage supernatants. The downregulation of inflammation in contact with the heparinized substrates induces higher expression of bone-related markers by MSCs.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2018. Vol. 7, no 5, article id 1700867
Keywords [en]
calcium phosphates; heparinization; inflammation; osteogenesis
National Category
Medical Materials Biomaterials Science
Research subject
Engineering Science with specialization in Materials Science
Identifiers
URN: urn:nbn:se:uu:diva-340741DOI: 10.1002/adhm.201700867ISI: 000426758500005OAI: oai:DiVA.org:uu-340741DiVA, id: diva2:1179728
Funder
The Swedish Foundation for International Cooperation in Research and Higher Education (STINT), STINT-IG2011-2047Available from: 2018-02-02 Created: 2018-02-02 Last updated: 2018-08-09Bibliographically approved

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The full text will be freely available from 2018-12-22 13:18
Available from 2018-12-22 13:18

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Lu, XiPersson, Cecilia

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