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Clotting protein: An extracellular matrix (ECM) protein involved in crustacean hematopoiesis
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology.
2018 (English)In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 78, p. 132-140Article in journal (Refereed) Published
Abstract [en]

Hematopoietic progenitor cells in crustaceans are organized in lobule-like structures surrounded by different types of cells and extracellular matrix (ECM) proteins in a Hematopoietic tissue (HPT). Here we show that the clotting protein (CP) is part of the ECM in HPT and is secreted during HPT cell culture. The formation of a filamentous network of CP was observed in HPT cell culture. A high amount of CP protein was detected at the surfaces of undifferentiated cells (round-shaped) compared with migrating cells (spindle shaped). Co-localization of the CP protein and TGase activity was observed on the cell surface and filamentous network between cells. A role for CP together with collagen was revealed in a 3D culture in which a collagen-I matrix was immobilized with CP or supplemented with CP. The results showed possible functions of CP, collagen, TGase and the cytokine Ast1 in the regulation of HPT progenitor cell behavior. This is the first study to provide insight into the role of CP, which probably not only participates in clot formation but also functions as an ECM component protein controlling hematopoietic stem cell behavior.

Place, publisher, year, edition, pages
2018. Vol. 78, p. 132-140
Keywords [en]
Clotting protein, ECM, Hematopoiesis, Crustacean
National Category
Zoology
Identifiers
URN: urn:nbn:se:uu:diva-340667DOI: 10.1016/j.dci.2017.09.017ISI: 000413881000014PubMedID: 28943319OAI: oai:DiVA.org:uu-340667DiVA, id: diva2:1180283
Funder
Swedish Research Council, 621-2012-2416Available from: 2018-02-05 Created: 2018-02-05 Last updated: 2018-02-05Bibliographically approved

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Junkunlo, KingkamonSöderhäll, KennethSöderhäll, Irene

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