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Quantitative Assessment of Drug Delivery to Tissues and Association with Phospholipidosis: A Case Study with Two Structurally Related Diamines in Development
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. Uppsala University, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-1557-4416
Grunenthal GmbH, D-52099 Aachen, Germany.
Grunenthal GmbH, D-52099 Aachen, Germany.
Fraunhofer Chalmers Ctr, Chalmers Sci Pk, S-41288 Gothenburg, Sweden.; Chalmers Univ Technol, Dept Math Sci, S-41296 Gothenburg, Sweden.; Univ Gothenburg, S-41296 Gothenburg, Sweden.
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2017 (English)In: Molecular Pharmaceutics, ISSN 1543-8384, E-ISSN 1543-8392, Vol. 14, no 12, p. 4362-4373Article in journal (Refereed) Published
Abstract [en]

Drug induced phospholipidosis (PLD) may be observed in the preclinical phase of drug development and pose strategic questions. As lysosomes have a central role in pathogenesis of PLD, assessment of lysosomal concentrations is important for understanding the pharmacokinetic basis of PLD manifestation and forecast of potential clinical appearance. Herein we present a systematic approach to provide insight into tissue-specific PLD by evaluation of unbound intracellular and lysosomal (reflecting acidic organelles) concentrations of two structurally related diprotic amines, GRT1 and GRT2. Their intratissue distribution was assessed using brain and lung slice assays. GRT1 induced PLD both in vitro and in vivo. GRT1 showed a high intracellular accumulation that was more pronounced in the lung, but did not cause cerebral PLD due to its effective efflux at the blood-brain barrier. Compared to GRT1, GRT2 revealed higher interstitial fluid concentrations in lung and brain, but more than 30-fold lower lysosomal trapping capacity. No signs of PLD were seen with GRT2. The different profile of GRT2 relative to GRT1 is due to a structural change resulting in a reduced basicity of one amino group. Hence, by distinct chemical modifications, undesired lysosomal trapping can be separated from desired drug delivery into different organs. In summary, assessment of intracellular unbound concentrations was instrumental in delineating the intercompound and intertissue differences in PLD induction in vivo and could be applied for identification of potential lysosomotropic compounds in drug development.

Place, publisher, year, edition, pages
2017. Vol. 14, no 12, p. 4362-4373
Keywords [en]
brain slice, drug induced phospholipidosis, intracellular concentration, lung slice, pharmacokinetics, unbound drug
National Category
Pharmacology and Toxicology
Research subject
Pharmaceutical Pharmacology
Identifiers
URN: urn:nbn:se:uu:diva-342077DOI: 10.1021/acs.molpharmaceut.7b00480ISI: 000417342400025PubMedID: 29099189OAI: oai:DiVA.org:uu-342077DiVA, id: diva2:1183688
Available from: 2018-02-19 Created: 2018-02-19 Last updated: 2018-03-08Bibliographically approved

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Loryan, IrenaHammarlund-Udenaes, Margareta

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