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Synechocystis PCC 6803 cells heterologously expressing bacterial tyrosine ammonia lyase can use exogenous tyrosine for p-coumaric acid production
Chulalongkorn Univ, Program Biotechnol, Fac Sci, Bangkok 10330, Thailand.;Chulalongkorn Univ, Dept Biochem, Fac Sci, Bangkok 10330, Thailand..
Naresuan Univ, Sci Lab Ctr, Fac Sci, Phitsanulok 65000, Thailand..
Naresuan Univ, Fac Allied Hlth Sci, Dept Med Technol, Phitsanulok 65000, Thailand..
Chulalongkorn Univ, Dept Biochem, Fac Sci, Bangkok 10330, Thailand..
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2018 (English)In: Journal of Plant Biochemistry and Biotechnology, ISSN 0971-7811, E-ISSN 0974-1275, Vol. 27, no 1, p. 118-122Article in journal (Refereed) Published
Abstract [en]

Phototrophic cyanobacteria may be considered as an alternative host for producing numerous bioactive compounds. We demonstrate that the Synechocystis PCC 6803 expressing tyrosine ammonia-lyase from Rhodobacter sphaeroides under Ptrc1O promoter produce p-coumaric acid at a rate three times higher than that under Ptrc1Ocore promoter, accounting for 18.4 +/- 1.5 mu g of p-coumaric acid per 10(8) cells (0.36 mg L-1). Additionally, our study is the first report to show the biotransformation of tyrosine to p-coumaric acid reaching a maximum 2.4-fold increase when 0.5 mM tyrosine was supplemented to the growth medium. Liquid chromatography-mass spectrometry analysis revealed the occurrence of diverse patterns of metabolites under different concentrations of supplemented tyrosine, suggesting that it is used in additional metabolic pathways.

Place, publisher, year, edition, pages
SPRINGER INDIA , 2018. Vol. 27, no 1, p. 118-122
Keywords [en]
Biotransformation, p-Coumaric acid, LC-MS analysis, Synechocystis PCC 6803, Tyrosine
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-342463DOI: 10.1007/s13562-017-0416-8ISI: 000422696800012OAI: oai:DiVA.org:uu-342463DiVA, id: diva2:1185555
Funder
Swedish Energy AgencyKnut and Alice Wallenberg FoundationAvailable from: 2018-02-26 Created: 2018-02-26 Last updated: 2018-02-26Bibliographically approved

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Lindblad, Peter

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