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Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine
Inst Laue Langevin, Life Sci Grp, 71 Ave Martyrs,BP 156, F-38042 Grenoble 9, France.;Malmo Univ, Fac Hlth & Soc, Biofilm Res Ctr Biointerfaces, S-20506 Malmo, Sweden.;Malmo Univ, Fac Hlth & Soc, Biomed Sci Dept, S-20506 Malmo, Sweden..
Malmo Univ, Fac Hlth & Soc, Biofilm Res Ctr Biointerfaces, S-20506 Malmo, Sweden.;Malmo Univ, Fac Hlth & Soc, Biomed Sci Dept, S-20506 Malmo, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
Inst Laue Langevin, Life Sci Grp, 71 Ave Martyrs,BP 156, F-38042 Grenoble 9, France..
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2018 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 34, no 1, p. 472-479Article in journal (Refereed) Published
Abstract [en]

Cholesterol is an essential component of mammalian membranes and is known to induce a series of physicochemical changes in the lipid bilayer. Such changes include the formation of liquid-ordered phases with an increased thickness and a configurational order as compared to liquid-disordered phases. For saturated lipid membranes, cholesterol molecules localize close to the lipid head group-tail interface. However, the presence of polyunsaturated lipids was recently shown to promote relocation of cholesterol toward the inner interface between the two bilayer leaflets. Here, neutron reflection is used to study the location of cholesterol (both non-deuterated and per-deuterated versions are used) within supported lipid bilayers composed of a natural mixture of phosphatidylcholine (PC). The lipids were produced in a genetically modified strain of Escherichia coli and grown under specific deuterated conditions to give an overall neutron scattering length density (which depends on the level of deuteration) of the lipids matching that of D2O. The combination of solvent contrast variation method with specific deuteration shows that cholesterol is located closer to the lipid head group-tail interface in this natural PC extract rather than in the center of the core of the bilayer as seen for very thin or polyunsaturated membranes.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2018. Vol. 34, no 1, p. 472-479
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
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URN: urn:nbn:se:uu:diva-343386DOI: 10.1021/acs.langmuir.7b02716ISI: 000422611500057PubMedID: 29232134OAI: oai:DiVA.org:uu-343386DiVA, id: diva2:1186322
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Swedish Research CouncilAvailable from: 2018-02-28 Created: 2018-02-28 Last updated: 2018-02-28Bibliographically approved

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