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Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma
NIST, Marine Biochem Sci Grp, Div Chem Sci, Hollings Marine Lab, Charleston, SC 29412 USA..
NIST, Stat Engn Div, Gaithersburg, MD 20899 USA..
NIST, Marine Biochem Sci Grp, Div Chem Sci, Hollings Marine Lab, Charleston, SC 29412 USA..
NIST, Marine Biochem Sci Grp, Div Chem Sci, Hollings Marine Lab, Charleston, SC 29412 USA..
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2017 (English)In: Journal of Lipid Research, ISSN 0022-2275, E-ISSN 1539-7262, Vol. 58, no 12, p. 2275-2288Article in journal (Refereed) Published
Abstract [en]

As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950-Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each laboratory using a different lipidomics workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detected in SRM 1950 serve as community-wide benchmarks for intra-and interlaboratory quality control and method validation. These analyses were performed using nonstandardized laboratory-independent workflows. The consensus locations were also compared with a previous examination of SRM 1950 by the LIPID MAPS consortium.jlr While the central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, it was also initiated to stimulate a discussion regarding areas in need of improvement.

Place, publisher, year, edition, pages
2017. Vol. 58, no 12, p. 2275-2288
Keywords [en]
fatty acyls, glycerolipids, lipids, phospholipids, quality control, quantitation, sphingolipids, Standard Reference Material 1950, sterols, National Institute of Standards and Technology
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-344222DOI: 10.1194/jlr.M079012ISI: 000416963900004PubMedID: 28986437OAI: oai:DiVA.org:uu-344222DiVA, id: diva2:1187840
Funder
Swedish Research Council, 2015-4870Swedish Heart Lung Foundation, HLF 20140469; HLF 20150640Available from: 2018-03-06 Created: 2018-03-06 Last updated: 2018-03-06Bibliographically approved

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Bergquist, JonasUbhayasekera, S. J. Kumari

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