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Comparisons between commercial salivary testosterone enzyme-linked immunosorbent assay kits
Orebro Univ, Dept Neurol, Fac Med & Hlth, Orebro, Sweden..
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.ORCID iD: 0000-0002-4597-041x
Linkoping Univ, Dept Clin Chem, Linkoping, Sweden.;Linkoping Univ, Dept Clin & Expt Med, Linkoping, Sweden..
Orebro Univ, Dept Neurol, Fac Med & Hlth, Orebro, Sweden.;Linkoping Univ, Dept Clin Chem, Linkoping, Sweden.;Linkoping Univ, Dept Clin & Expt Med, Linkoping, Sweden..
2017 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 77, no 8, p. 582-586Article in journal (Refereed) Published
Abstract [en]

Introduction: Measuring testosterone concentrations is of interest both in clinical situations and for research, the latter expanding rapidly during recent years. An increased demand for convenient methods has prompted a number of companies to develop enzyme-linked immunosorbent assay (ELISA) kits to measure testosterone concentrations in saliva. However, the inter-comparability of kits from different manufacturers have yet to be determined. Aim of study: The aim of this study was to compare commercially available ELISA kits from four different manufacturers (Salimetrics, IBL, DRG and Demeditec). Methods: Saliva was collected from 50 participants (25 men and 25 women). Each sample was analysed by the four ELISA kits. Results: The correlations between the ELISA kits from Demeditec, DRG and Salimetrics were moderate to high with r-values >.77; however, proportional errors between the methods calls for caution. The ELISA kit from IBL malfunctioned and no results from this kit was obtained. Conclusions: Results from studies using the ELISA kits from Demeditec, DRG and Salimetrics are generally comparable; however, translation using the formulae presented in the current study could increase the accuracy of these comparisons.

Place, publisher, year, edition, pages
2017. Vol. 77, no 8, p. 582-586
Keywords [en]
Androgens, immunoassay, methods, saliva, testosterone
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-343341DOI: 10.1080/00365513.2017.1339231ISI: 000416756100004PubMedID: 28644096OAI: oai:DiVA.org:uu-343341DiVA, id: diva2:1189565
Funder
Swedish Research Council, 2015-4870Available from: 2018-03-12 Created: 2018-03-12 Last updated: 2018-03-15Bibliographically approved

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