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RNAlater (R) is a viable storage option for avian influenza sampling in logistically challenging conditions
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.ORCID iD: 0000-0002-5629-0196
Department of Laboratory Medicine, Division of Clinical Microbiology, Dalarna County, Sweden.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Capital Med Univ, Xuanwu Hosp, Beijing, Peoples R China..
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2018 (English)In: Journal of Virological Methods, ISSN 0166-0934, E-ISSN 1879-0984, Vol. 252, p. 32-36Article in journal (Refereed) Published
Abstract [en]

Surveillance of wild birds is critical in monitoring for highly pathogenic avian influenza A viruses (ATVs). However, a successful surveillance regime requires proper treatment of samples in the field - rapid placement of samples in -80 degrees C and subsequent maintenance of cold-chain. Given the logistical difficulties of this, many avian taxa and/or geographic locations are not sampled, or, when sampled may result in false negatives due to poor sample treatment in the field. Here, we assessed the utility of RNAlater (R) as a stabilization agent for AIV sampling. We found no difference in real time PCR performance between virus transport media at optimal conditions and RNAlater (R) at -80 degrees C, -20 degrees C, 4 degrees C or room temperature up to two weeks, at either low or high virus load. Not only was RNAlater (R) useful in comparison of spiked samples or those from duck experiments, it was employed successfully in a field study of backyard birds in China. We detected AIV in cloacal and oropharyngeal samples from chickens and a sample with a low Cq was successfully subtyped as H9, although sample storage conditions were suboptimal. Thus, despite limitations in downstream characterization such virus isolation and typing, RNAlater (R) is a viable option for AIV sampling under logistically challenging circumstances.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2018. Vol. 252, p. 32-36
Keyword [en]
China, Influenza A virus, RNAlater (R), RNA preservative, Storage conditions, VTM
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-345720DOI: 10.1016/j.jviromet.2017.11.004ISI: 000423889000005PubMedID: 29129490OAI: oai:DiVA.org:uu-345720DiVA, id: diva2:1190758
Funder
Swedish Research Council Formas, 2011-2013-1320, 2016-00790Swedish Research Council, 2016-02606
Available from: 2018-03-15 Created: 2018-03-15 Last updated: 2018-03-15Bibliographically approved

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Lundkvist, ÅkeJärhult, Josef D.

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