uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Electrochemical detection of influenza virus H9N2 based on both immunomagnetic extraction and gold catalysis using an immobilization-free screen printed carbon microelectrode
Institut Pasteur de Tunis, LR11IPT03, Laboratory of Epidemiology and Veterinary Microbiology (LEMV), Tunis-Belvédère 1002, Tunisia.; Faculté des Sciences de Tunis, Campus Universitaire El-Manar, 2092 El Manar, Tunis, Tunisia.;Université Tunis El Manar, Tunis 1068, Tunisia.
Institut Pasteur de Tunis, LR11IPT03, Laboratory of Epidemiology and Veterinary Microbiology (LEMV), Tunis-Belvédère 1002, Tunisia.; Prince Sattam Bin Abdulaziz University, Saudi Arabia.
Institut Pasteur de Tunis, LR11IPT03, Laboratory of Epidemiology and Veterinary Microbiology (LEMV), Tunis-Belvédère 1002, Tunisia.;Université Tunis El Manar, Tunis 1068, Tunisia.
Institut Pasteur de Tunis, LR11IPT03, Laboratory of Epidemiology and Veterinary Microbiology (LEMV), Tunis-Belvédère 1002, Tunisia.;Université Tunis El Manar, Tunis 1068, Tunisia.
Show others and affiliations
2018 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 107, p. 170-177Article in journal (Refereed) Published
Abstract [en]

Influenza is a viral infectious disease considered as a source of many health problems and enormous socioeconomic disruptions. Conventional methods are inadequate for in-field detection of the virus and generally suffer from being laborious and time-consuming. Thus, studies aiming to develop effective alternatives to conventional methods are urgently needed. In this work, we developed an approach for the isolation and detection of influenza A virus subtype H9N2. For this aim, two specific influenza receptors were used. The first, anti-matrix protein 2 (M2) antibody, was attached to iron magnetic nanoparticles (MNPs) and used for the isolation of the virus from allantoic fluid. The second biomolecule, Fetuin A, was attached to an electrochemical detectable label, gold nanoparticles (AuNPs), and used to detect the virus tacking advantage from fetuin-hemagglutinin interaction. The MNP-Influenza virus-AuNP formed complex was isolated and treated by an acid solution then the collected gold nanoparticles were deposited onto a screen printed carbon electrode. AuNPs catalyzes the hydrogen ions reduction in acidic medium while applying an appropriate potential, and the generated current signal was proportional to the virus titer. This approach allows the rapid detection of influenza virus A/H9N2 at a less than 16 HAU titer.

Place, publisher, year, edition, pages
2018. Vol. 107, p. 170-177
Keywords [en]
Influenza Virus A, H9N2, Biosensors, Nanoparticles, Chronoamperometry
National Category
Analytical Chemistry Nano Technology
Research subject
Chemistry with specialization in Analytical Chemistry; Chemistry with specialization in Bioorganic Chemistry; Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
URN: urn:nbn:se:uu:diva-346280DOI: 10.1016/j.bios.2018.02.018ISI: 000428006900020PubMedID: 29455027OAI: oai:DiVA.org:uu-346280DiVA, id: diva2:1190974
Funder
EU, Horizon 2020, 645758Available from: 2018-03-16 Created: 2018-03-16 Last updated: 2018-06-20Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Authority records BETA

Österlund, Lars

Search in DiVA

By author/editor
Österlund, Lars
By organisation
Solid State Physics
In the same journal
Biosensors & bioelectronics
Analytical ChemistryNano Technology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 66 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf