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Strategies for analysis of isomeric peptides
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.ORCID iD: 0000-0002-0675-3412
2018 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 41, no 1, p. 385-397Article, review/survey (Refereed) Published
Abstract [en]

This review presents an overview and recent progress of strategies for detecting isomerism in peptides, with focus on D/L epimerization and the various isomers that the presence of an aspartic acid residue may yield in a protein or peptide. While mass spectrometry has become a majorly used method of choice within proteomics, isomerism is inherently difficult to analyze because it is a modification that does not yield any change in mass of the analyte. Here, several techniques used for analysis of peptide isomerism are discussed, including enzymatic assays, liquid chromatography, and capillary electrophoresis. Recent progress in method development using mass spectrometry is also discussed, including labeling strategies, fragmentation techniques, and ion-mobility spectrometry.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2018. Vol. 41, no 1, p. 385-397
Keywords [en]
aspartic acid, chromatography, D-amino acids, mass spectrometry, proteomics
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-347108DOI: 10.1002/jssc.201700852ISI: 000419514100020PubMedID: 28922569OAI: oai:DiVA.org:uu-347108DiVA, id: diva2:1193159
Funder
Swedish Research Council, 2015-00406Swedish Foundation for Strategic Research , ICA16-0010Available from: 2018-03-26 Created: 2018-03-26 Last updated: 2018-05-04Bibliographically approved

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Jansson, Erik T.

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