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Continuous root xylem formation and vascular acclimation to water deficit involves endodermal ABA signalling via miR165
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Physiological Botany. Linnean Ctr Plant Biol, Ullsv 24E, SE-75651 Uppsala, Sweden.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Physiological Botany. Linnean Ctr Plant Biol, Ullsv 24E, SE-75651 Uppsala, Sweden.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Physiological Botany. Linnean Ctr Plant Biol, Ullsv 24E, SE-75651 Uppsala, Sweden.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Physiological Botany. Linnean Ctr Plant Biol, Ullsv 24E, SE-75651 Uppsala, Sweden, Canada..
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2018 (English)In: Development, ISSN 0950-1991, E-ISSN 1477-9129, Vol. 145, no 3, article id dev159202Article in journal (Refereed) Published
Abstract [en]

The plant root xylem comprises a specialized tissue for water distribution to the shoot. Despite its importance, its potential morphological plasticity in response to environmental conditions such as limited water availability has not been thoroughly studied. Here, we identify a role for the phytohormone abscisic acid (ABA) for proper xylem development and describe how ABA signalling-mediated effects on core developmental regulators are employed to alter xylem morphology under limited water availability in Arabidopsis. Plants with impaired ABA biosynthesis and reduced ABA signalling in the cell layer surrounding the vasculature displayed defects in xylem continuity, suggesting that non-cell autonomous ABA signalling is required for proper xylem development. Conversely, upon external ABA application or under limited water availability, extra xylem strands were formed. The observed xylem developmental alterations were dependent on adequate endodermal ABA signalling, which activated MIR165A. This resulted in increased miR165 levels that repress class III HD-ZIP transcription factors in the stele. We conclude that a pathway known to control core developmental features is employed as a means of modifying plant xylem morphology under conditions of environmental stress.

Place, publisher, year, edition, pages
COMPANY OF BIOLOGISTS LTD , 2018. Vol. 145, no 3, article id dev159202
Keywords [en]
ABA, Arabidopsis thaliana, HD-ZIP III transcription factors, miR165, Xylem
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-347084DOI: 10.1242/dev.159202ISI: 000424653300014OAI: oai:DiVA.org:uu-347084DiVA, id: diva2:1193717
Available from: 2018-03-27 Created: 2018-03-27 Last updated: 2020-01-10Bibliographically approved
In thesis
1. Stelar Performance Under Drought: Regulation of Developmental Robustness and Plasticity of the Arabidopsis Root Xylem
Open this publication in new window or tab >>Stelar Performance Under Drought: Regulation of Developmental Robustness and Plasticity of the Arabidopsis Root Xylem
2020 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Plants have evolved genetic mechanisms to sense, modulate and modify developmental programs in response to the changing environment. This brings forth challenges in stably generating tissue patterns while simultaneously allowing amenability. Gene perturbation studies have identified molecular regulators that control fate specification and differentiation of various tissues. However, we lack a complete understanding of how these processes are influenced by the environment. In this thesis, using Arabidopsis xylem as a model, I show that developmental regulators that function in maintaining a stable growth pattern are also involved in the manifestation of phenotypic plasticity. We found that the generation of a robust xylem developmental program is dependent on a feed forward loop between components of the auxin signalling pathway and the master regulators of xylem development, class III Homeodomain Leucine-Zipper (HD-ZIP III) transcription factors (TFs). By directly activating an auxin signalling activator (MP) and repressor (IAA20), the HD-ZIP III TFs facilitate stable xylem patterning and development. We also show that alterations to the HD-ZIP III mediated xylem developmental program were caused non-cell autonomously by changes in levels and signalling of a key regulator of abiotic stress response, abscisic acid (ABA). The suppression and enhancement of ABA signalling resulted in lower and higher levels respectively of mir165, a known post transcriptional regulator of HD-ZIP III levels. Under conditions of enhanced ABA signalling we found that ABA also acts cell autonomously through master regulators of xylem differentiation, VASCULAR RELATED NAC-DOMAIN (VND) transcription factors. Furthermore, we show that both cell autonomous and non-cell autonomous pathways are employed during water deficit conditions to alter xylem morphology and differentiation rate, likely to enhance water uptake. Taken together, our results show that ABA’s influence on evolutionarily conserved development regulators is important for xylem developmental plasticity. The identification of genetic regulators that control plant phenotypic alterations to limited water availability such as those identified in this thesis will be important to develop tolerant varieties that can survive the extended periods of drought caused by the alarming rise in global temperatures.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2020. p. 69
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1894
Keywords
HD-ZIP III TFs, miR165, Xylem, ABA, VND, plasticity, water deficit
National Category
Natural Sciences Agricultural and Veterinary sciences
Research subject
Biology with specialization in Physiological Botany
Identifiers
urn:nbn:se:uu:diva-401800 (URN)978-91-513-0847-0 (ISBN)
Public defence
2020-02-28, A-281 Lennart Kennes Sal, Uppsala Biocenter, Almas Allé 5, Uppsala, 13:00 (English)
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Supervisors
Available from: 2020-02-06 Created: 2020-01-10 Last updated: 2020-02-06

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Ramachandran, PrashanthCarlsbecker, Annelie

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