uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
PRESERVATION OF DNA AND BACTERIA FOR INCORPORATION IN CULTURE COLLECTIONS
2015 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

This study contains of two different parts, an investigation of long time stored prokaryotic DNA for incorporation in a DNA-bank, and lyophilization of two Flavobacterium strains with different lyoprotectants and validation of an alternative method for determination of concentration. For the DNA-project PCR and capillary gel electrophoresis was used for sequencing, and lyophilization, CFU-counts and Start of growth time was used to investigate the Flavobacteriums. 87.5% of the DNA samples with maintained quality were correctly identified. The lyophilization was successful, with varying results from the revivals. The validation could not be completed due to incoherent results between the two methods.An alternative validation method is suggested. The presence of viable but non-cultivable should also be considerated for continued studies.

Place, publisher, year, edition, pages
2015.
Keywords [en]
Lyophilization, Freeze-drying, DNA-bank, Sequencing, Marmur method.
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349002OAI: oai:DiVA.org:uu-349002DiVA, id: diva2:1199161
External cooperation
Ghent University
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Available from: 2018-04-20 Created: 2018-04-19 Last updated: 2018-04-20Bibliographically approved

Open Access in DiVA

No full text in DiVA

Biomedical Laboratory Science/Technology

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 24 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf