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GENETIC CHARACTERIZATION AND DISINFECTANT SUSCEPTIBILITY TESTING OF VEROTOXIN-PRODUCING Escherichia coli O157:H7 FROM SWEDISH CATTLE
2015 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Background Verotoxin-producing Escherichia coli (VTEC) O157:H7 is a common cause of intestinal infections in humans. Cattle is the main reservoir, and food or water contaminated by animal feces are common sources of infection. The symptoms are usually bloody diarrhea and abdominal pain, and complications as haemolytic uremic syndrome (HUS) may occur. Chlorhexidine is a widely used disinfectant both in veterinary and human medicine. Treatments using chlorhexidine have shown to be effective in reducing the fecal shedding of VTEC O157:H7.Aim The aim of this study was to genetically characterize and type a selection of VTEC O157:H7 isolates found in Swedish cattle. Additionally, the purpose was to determine if four major subgroups of VTEC O157:H7 show different levels of susceptibility against chlorhexidine.Material and methods The E. coli strains analysed were isolated from fecal samples of cattle. The characterization method applied was next generation sequencing, and the susceptibility test was performed using the broth macrodilution method.Results The results showed that the prevalence of the most virulent subgroup has decreased compared to past years. The susceptibility test showed no significant difference between the four subgroups.Conclusion The results obtained in this study have improved the knowledge about the strains currently present in the country, which facilitates the monitoring and control processes. The data obtained shows that the prevalence of the most pathogenic subgroup has decreased. The major subgroups of VTEC O157:H7 have similar levels of susceptibility against chlorhexidine, which is useful information as chlorhexidine is a widely used disinfectant.

Place, publisher, year, edition, pages
2015.
Keywords [en]
Next-generation sequencing, LSPA-6, clade 8, chlorhexidine, minimum inhibitory concentration
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349004OAI: oai:DiVA.org:uu-349004DiVA, id: diva2:1199168
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-20 Created: 2018-04-19 Last updated: 2018-04-20Bibliographically approved

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