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Standardization of the method volume determination of separation media using dry scale analysis
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
2015 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

GE Healthcare Life sciences is a global company known worldwide for their separation media products. They provide different types of products for biochemical analyzes. Earlier studies have indicated that different variables may have an impact on the method volume determination of separation media.This study was performed to standardize the method.Different parameters such as vacuum, type of gel slurry, amount of acetone and liquid trap were examined to determine their impact on the method. In addition, three different products were tested. The product that was tested was concentrated to a specific gel slurry and then poured into a cube, which was used to determinate the volume of the separation media. Vacuum was then applied to the cube and the liquid from the product that had been poured into the cube was drained into the liquid trap. All experiments were analyzed by use of Minitab 16 and Excel.Only two variables showed significant impact on the method that was analyzed, gel slurry and product type. The amount of acetone used impacted the dry time of the separation media. The liquid trap and vacuum didn’t show any impact on the method. The time of the vacuum in the cube needs to be further examined.This confirms that the method volume determination of separation media using dry scale analysis is a good method with few error sources.

Place, publisher, year, edition, pages
2015.
Keyword [en]
Vacuum, Cube, Gel slurry, Acetone, Agarose
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349010OAI: oai:DiVA.org:uu-349010DiVA, id: diva2:1199220
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-20 Created: 2018-04-19 Last updated: 2018-04-20Bibliographically approved

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