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The detection of dermatophytes in clinical samples, by PCR and cultivation, afterprolonged storage in 4°C.
2016 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Dermatomycosis is a superficial fungal infection that affects both humans and animals and is caused by either yeasts or filamentous fungi, also called dermatophytes. The dermatophytes is keratinofilic organisms that attacks tissue made of keratin such as skin, hair and nails. It’s one of the most infectious diseases in human with a global prevalence of 20-25 %. Even though the high prevalence, the research of dermatophytes lacks of studies where clinical samples has been long-term stored in cold condition, approximately 4ºC. This study evaluates the viability and detection of dermatophytes in stored samples. The results could make way of benchmarks for storage of clinical samples suspected of dermatomycosis.A total of 171 clinical samples, consisted of skin, hair and nails, has been collected from dermatology clinics at Uppsala University Hospital and Karolinska University Hospital from 2013-2015. One set of samples was sent to a mycology laboratory for diagnosis while its twin sample was analysed by use of direct microscopy and then stored in 4ºC. The stored samples were analysed by either cultivation or PCR.Viability of the dermatophytes after cultivation was estimated to 35 %, where T. rubrum showed the greatest loss of viability. The PCR analysis detect 85 % of all analysed samples, all of them were identified as T. rubrum.The results indicate that dermatophytes, especially T. rubrum, had the greatest loss in viability after long-term storage in 4ºC. Therefore PCR, with its ability to detect dead material, showed better performance and is the preferred for analysis of stored dermatophytes.

Place, publisher, year, edition, pages
2016.
Keyword [en]
Dermatomycosis, preservation, T. Rubrum, viability, agar
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349016OAI: oai:DiVA.org:uu-349016DiVA, id: diva2:1199236
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-20 Created: 2018-04-19 Last updated: 2018-04-20Bibliographically approved

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