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Comparison of Qiasymphony SP and QuickGene for extraction and purification of DNA
2016 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

The genome is inherited information encoded in the organism's whole DNA sequence. In the human body, genome corresponding to the genetic material is found in all cells except erythrocyte.DNA from different cells can be obtained by DNA extraction, which is the fundamental method for most DNA-based analyzes and can be used to investigate hereditary diseases. The objective of this project was to compare DNA extraction with QIAsymphony and Quick Gene, which is an old extraction robot and, the number of samples that can be handled per run is limited. Therefore, this robot will be replaced by QIAsymphony, which has a greater capacity than Quick Gene and is more efficient and also automatic.During the project, the extraction of blood was performed in parallel on the current extraction robot Quick Gene and the QIAsymphony SP. After extraction, DNA quality was tested by measuring the DNA concentration and thereby obtaining ration for A260 / 280 and A230/260 with NanoDropTM. Furthermore, DNA quality was also analyzed by using Tape Station. To measure if DNA quality is affected over time, samples were extracted in parallel on both instruments after 0, 1, 3, 5 and 7 days.In general, the results of the analysis of the extracted samples on the QIAsymphony SP showed that this method is comparable or better than samples extracted by Quick Gene. QIAsymphony robot is much more versatile for clinical genetics compared to Quick Gene robot because it require less manual handling which reduces both hands-on time and cost.

Place, publisher, year, edition, pages
2016.
Keywords [en]
DNA purity, extraction, genetics, magnetic ball, Nanodrop
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349017OAI: oai:DiVA.org:uu-349017DiVA, id: diva2:1199239
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-20 Created: 2018-04-19 Last updated: 2018-04-20Bibliographically approved

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