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COMPARING FREEZING MEDIUM FOR SEMEN IN TERMS OF MOTILITY, CONCENTRATION AND BIOCHEMISTRY: ORIGIO VERSUS NIDACON VERSUS VITROLIFE
2016 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Due to infertility among 10 % of couples, the Swedish government has voted for a tax covered in vitro fertilization (IVF) treatment for infertile couples since infertility is referred as a disability. Depending on the sperm quality and concentration, the treatment of IVF is either insemination or intracytoplasmatic sperm injection (ICSI). If the sperm is of high concentration and quality, conventional insemination is usually sufficient, whilst low concentration and quality requires ICSI.The aim of this project was to compare three cryo media from three different companies, Origio, Nidacon and Vitrolife. The outcomes were sperm mortality, motility and concentration. An additional project of this study was to determine if three biomedical markers, a-glucosidase, fructose and zinc, differed between sperm samples. The same sperm samples were used both for cryo media testing and concentration of biomedical markers in relation to sperm survival. After freezing in Nidacon media, slightly more motile sperms were seen after thawing than after freezing in the other two cryo media, but the difference was not significant. The average survival rate of sperms after freezing was 6.4 %, which was critically lower than expected. The conclusion of the biochemical project did not show any difference between sperm survival and concentration of the biochemical markers, thus leaving us with a probability that the biochemistry of the male reproduction organ has no effect on the sperm survival when used in freezing and thawing process.

Place, publisher, year, edition, pages
2016.
Keywords [en]
cryopreservation, IVF, ICSI, gradient preperation, sperm motility
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349019OAI: oai:DiVA.org:uu-349019DiVA, id: diva2:1199240
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-20 Created: 2018-04-19 Last updated: 2018-04-20Bibliographically approved

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