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Comparison of Current and Modified Flotation and Centrifugation Method and Validation of Real-time PCR for Detection of A. perfoliata, Cyathostominae and S. vulgaris
2016 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Equine endoparasites are organisms that use horses as their hosts. Equine parasites that occur frequently are pinworms (Oxyuris equi), small strongyle (Cyathostominae), large strongyle (Strongylus vulgaris), tapeworm (Anoplocephala spp) and roundworm (Parascaris equorum). The most pathogenic parasite in horses is S.vulgaris which can cause necrosis of the intestinal mucosa.The aim of the study was to compare the current flotation- and centrifugation method used at the parasitology laboratory at National Veterinary Institute (NVI) with a modified flotation- and centrifugation method. The second aim was to perform a validation of a real-time PCR for detection of A.perfoliata, Cyathostominae and S.vulgaris. Faecal material that was provided came from horses of various ages from different regions in Sweden.The comparison between the flotation- and centrifugation methods showed no significant difference in assessment in the light microscope. However, the modified method is far less time consuming. Results from the study showed that real-time PCR had a detection limit of two eggs for S.vulgaris. The relationship between Ct value and the number of eggs gave R2=0,931, which indicated a strong correlation.The study showed that the modified method reduces analysis time considerably compared to the current flotation- and centrifugation method. The validation of real-time PCR demonstrated that it can detect endoparasites, both qualitatively and quantitatively.

Place, publisher, year, edition, pages
2016.
Keyword [en]
Equine endoparasites, Cestodes, Larval cultures, TaqMan, SYBR Green
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349037OAI: oai:DiVA.org:uu-349037DiVA, id: diva2:1199282
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-20 Created: 2018-04-20 Last updated: 2018-04-20Bibliographically approved

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