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Verification of Bordetella pertussis and Bordetella holmesii withreal-time PCR
2017 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Bordetella pertussis causes whooping cough, which is a very dangerous disease especially for children. A correct diagnose is very important. Bordetella pertussis usually misidentifies with Bordetella holmesii by routine polymerase chain reaction.The aim of the study is to evaluate a target sequence that can verify a Bordetella pertussis infection and distinguish it from other Bordetella species, in specific Bordetella holmesii.The target sequences' sensitivities were tested on the control bacterial strains. Sixty-five clinical samples were analyzed with real-time PCR.The study concludes that both IS481 and ptxA target sequences are highly specific to detect B. pertussis, while both ho_IS1001 and IS481 are specific to detect B. holmesii. Pa_IS1001 is specific to detect B. parapertussis. Culturing is less sensitive to detect Bordetella infection than real-time PCR.

Place, publisher, year, edition, pages
2017.
Keywords [en]
Pertussis toxin, identification, B. paraertussis, IS481 sequence, IS1001 sequence.
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349077OAI: oai:DiVA.org:uu-349077DiVA, id: diva2:1199382
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-20 Created: 2018-04-20 Last updated: 2018-04-20Bibliographically approved

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