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Effects of the different surface nanotopography of Poly(MAA-DAP) on the activation of the cascade systems in blood
2017 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Background: Biomaterials are used for transplants and dialyses. A biomaterial that is not well adjusted to the body but is inserted into the body, may cause serious thrombosis and thromboembolic events as well as inflammation. PVC hoses used in dialysis today causes substantial complement activation. Therefore, poly (MAA-DAP), a polymer that has been developed in to four different nanotopographies, could be a possible biomaterial used in haemodialysis and catheters.Aim: The aim of these nanostructures is to see if it can be used as a substitute for PVC, and to see if there is a difference between the four different structures. Thus, these nanotopographies should have a low thrombogenic effect and a low complement activation.Material and methods: To analyse the compatibility with the cascade system, the intrinsic pathway and the alternative pathway, blood samples were analysed by use of ELISA. Plasma was collected from the 2-well slide chamber blood experiments were the whole blood was added to a slide chamber. The amount of platelets was, in addition to ELISA, also analysed using Sysmex XP-300 cellcounter.Results: The results showed that the MAA-DAP polymer surfaces were complement activating but not platelet activating. There was no significant difference in complement activation between the different MAA-DAP polymer surfaces. The more structured wires and fibrils were slightly more platelet activating but the difference was not significant enough.Conclusion: It can be concluded that the nanotopographies do not have significant impact on blood, it is the polymer material that effects the blood. Future studies could be performed on other type of material with different topographies and with blood.

Place, publisher, year, edition, pages
2017.
Keywords [en]
whole blood, platelets, complement system, contact activation, intrinsic pathway
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349078OAI: oai:DiVA.org:uu-349078DiVA, id: diva2:1199389
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-20 Created: 2018-04-20 Last updated: 2018-04-20Bibliographically approved

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