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Real-time PCR detection of MRSA on the CepheidGeneXpert®System
2017 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Background: MRSA or "Meticillin-resistant Staphylococcus aureus" has become a healthcareissue around the world. Over the last few years, MRSA infections has increased in 520 casesand become more common in society, causing a life threating infection and healthcareproblem. In order to avoid spreading of MRSA between patients in hospitals, specific analysisare necessary to monitor the bacteria.Aim: The purpose of the project was to validate NxG-kit in GeneXpert-instruments toverify and compare this kit to the Nasal Complete kit and to SYBR-Green based on in-housereal-time PCR. This comparison was made to see if NxG-kits can detect the SCCmec carryingMRSA's mecA and mecC-gene to replace in-house PCR and Nasal Complete-kits with NxGkits.Material and methods: In this project, some analyses such as in-house real time PCR andGeneXpert systems were performed using patient’s samples from different locations includingsome strains to detect specific genes in MRSA at the microbiology laboratory (Unilabs) inStockholm.Result: The result of the analysis performed with NxG-kit in GeneXpert-instruments hasmore potential to detect of positive MRSA samples compared to Nasal Complete-kit and inhousePCR.Conclusion: NxG-kit could easily detect the presence of MRSA because the kit targetsSCCmec carrying mecA- or mecC-gene in the orfX-gene, which provides accurate and rapidMRSA detection.

Place, publisher, year, edition, pages
2017.
Keywords [en]
mec-gene, SCCmec-element, PBP2a, PCR, Cepheid-GeneXpert
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349079OAI: oai:DiVA.org:uu-349079DiVA, id: diva2:1199391
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-20 Created: 2018-04-20 Last updated: 2018-04-20Bibliographically approved

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