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A new point-of-care-testing for blood typing using spectrophotometry
2017 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Blood groups are classified by presence or absence of certain antigens on the erythrocytes. Before a blood transfusion, there is a need to test the blood group of both the donor and the receiver. A simple test is needed for developing countries with low resources. Therefore, a new cuvette method that uses iron shavings to collect agglutinated red cells was developed, and the aim was to create a point of care blood typing test that is based on spectrophotometry.The method was first optimized. Blood samples from volunteers with different blood groups were collected and reagents, incubation time and stirring mode was determined. Levels for positive and negative sample was assessed. Blood sample stability test and a comparison with routine diagnostics were performed. Correlation between OD and Hb as well as OD and RBC-value was statistically calculated.It was possible to determine different blood groups in whole blood. Samples were stable for 5 days and the p-value for significant difference between positive and negative result all days was p<0.0001. The new method corresponds with the routine method used today.During the development of this test, it was noted that the erythrocytes needed to be handled carefully together with the iron shavings. The test was stable and it is possible that it could be performed by people with just little laboratory experience.This newly developed POC-test adapted for developing countries with low resources, leads to an interest in production for the clinical chemistry industries.

Place, publisher, year, edition, pages
2017.
Keywords [en]
Blood groups, immunohematology, erythrocytes, transfusion medicine, haemagglutination
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349090OAI: oai:DiVA.org:uu-349090DiVA, id: diva2:1199410
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-20 Created: 2018-04-20 Last updated: 2018-04-20Bibliographically approved

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