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Investigating pneumococcal loads and serotypes found on the nasopharynx of children with pneumonia and healthy children in The Gambia.Elina Garcia
2017 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Introduction: Streptococcus pneumoniae is a bacterium commonly found in the nasopharynx of young children. It can cause meningitis and pneumonia in children and left untreated, it can eventually lead to death, which is common in developing countries. The Gambia is a country that suffers from high rates of paediatric pneumonia, and is part of a WHO surveillance.Aim: The aim of the study was to compare and investigate pneumococcal loads and serotypes found in the nasopharynx of pneumonia patients to those in healthy controls. Methods: Nasopharyngeal swabs were collected from 115 healthy children and 31 children with pneumonia. Samples were tested for pneumococcus using real-time quantitative PCR. Positive qPCR samples were cultured and serotyped using microarray or latex agglutination. Result: Over 60 % (70 of 115) of healthy children had pneumococci in the nasopharynx compared to 94 % (29 of 31) of children with pneumonia. The pneumococcal loads were significant higher in pneumonia patients compared to healthy controls (p=0.0113), with different serotypes distribution observed between the two groups. Discussion: We identified significant differences in pneumococcal carriage rates, and densities between children with pneumonia and healthy controls. These findings are relevant to our understanding of pneumococcal disease and may aid in the development of new diagnostics.

Place, publisher, year, edition, pages
2017.
Keywords [en]
Streptococcus pneumoniae, qPCR, serotyping, surveillance, nasopharyngeal
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349093OAI: oai:DiVA.org:uu-349093DiVA, id: diva2:1199419
External cooperation
Murdoch Children’s Research Institute, Melbourne
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-20 Created: 2018-04-20 Last updated: 2018-04-20Bibliographically approved

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