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Evaluation and comparison of bactericidal effects between non-pathogen reduced and pathogen reduced human platelet concentrates
2017 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

In the blood, there are small cell fragments called platelets that activates hemostasis and contributes to the innate host defense. Platelets are said to have bactericidal effect such as storage of antibacterial peptides in their granules. In this study, we compared bactericidal effects of platelets before and after pathogen reduction by INTERCEPTâ„¢ system. Bacillus subtilis and Klebsiella pneumoniae were grown to log-phase in broth. Rickettsia helvetica was grown in cells. Bacillus subtilis at 108 CFU/ml, Klebsiella pneumoniae at 107 CFU/ml and Rickettsia helvetica (recovered from lysed cells) were incubated with both non-pathogen reduced and pathogen reduced platelet concentrates. Bacteria and platelets were incubated on slides, colored with LIVE/DEAD BacLightâ„¢ and detected with a fluorescence microscope. Bacterias and platelets were also incubated in tubes and grown on blood agar plates for different length of time. The plates were incubated overnight and read. Due to failure to optimize the fluorescence method, no results were received. Death curves for Bacillus subtilis before pathogen reduction showed decreased number of colonies after 1 h and after pathogen reduction the decrease was seen after 2 h. Both types of platelet concentrates showed non-readable results after 4 h. Death curves for both types of platelet concentrate with Klebsiella pneumoniae showed that the decreased survival started immediately after the bacteria incubation with the platelets was initiated. The platelets showed later activation when pathogen reduced. In summary, there was no major bactericidal effects on platelets.

Place, publisher, year, edition, pages
2017.
Keywords [en]
antibacterial, intercept system, granule, calcium gluconate, aggregation
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349095OAI: oai:DiVA.org:uu-349095DiVA, id: diva2:1199426
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-20 Created: 2018-04-20 Last updated: 2018-04-20Bibliographically approved

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CiteExportLink to record
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  • apa
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  • de-DE
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  • en-US
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  • nn-NB
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Output format
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