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Detection of splice variants by antibody-based proteomics methods
2017 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

The splice variants of the protein play important role in the discovery of different diseases cause. Some of protein isoforms share very high similarity in the sequence but other protein isoforms have low similarity in the sequence. Low similarity isoforms were easily more characterized than high similarity isoforms. The purpose of our study was to detection of different protein splice variants. In this study we have focused on identify the splice variants of minichromosome maintenance 3 (MCM3) protein using Western Blotting and Reverse Transcriptase Poly Chain Reaction (RT-PCR). By mono-specific antibodies and Western Blotting technique two splices were detected in various human tissues lysate. We couldn’t identify a third splice by Western Blotting technique because 1st and 3th protein isoforms have very small different between their molecular weights. All splice variants were detected separately by RT-PCR. Data from this study indicates that detection of splice variants by both techniques and helps annotate with more specific information protein splice variants.

Place, publisher, year, edition, pages
2017.
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349102OAI: oai:DiVA.org:uu-349102DiVA, id: diva2:1199452
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-20 Created: 2018-04-20 Last updated: 2018-04-20Bibliographically approved

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CiteExportLink to record
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