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OPTIMIZATION OF CRYOPRESERVATION METHODSFOR HUMAN SPERMATOZOA
2017 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

It is known that, in cryopreservation of gametes, decrease in post-thaw motility of the said cells remain obvious. Ice crystal formation is main cause for this, reason being that over 80 % of our cells consists of water. When cells are exposed to cryogenic temperatures below 0°C, ice formation begins. To minimise this problem, freezing medium known as cryoprotectant agents (CPAs) are used.The main aim of the present study was to evaluate two cryopreservation methods for human spermatozoa in terms of progressive sperm motility after one freeze-thaw cycle.Semen samples (n=49) were divided in two main groups, prepared samples (density gradient technique) and unprocessed samples. Sperm analysis was performed before freezing and after thawing. The samples were cryopreserved using method instructions from Origio and Nidacon companies. Both cryopreservation methods were compared by use of a paired student t-test which was done with the help of Microsoft excel software. A p-value <0.05 was considered statistically significant.Results indicated that samples processed prior to freezing gave a higher post-thaw yield. However, it was noted that samples which were frozen using Nidacon CPA surpassed Origio group (p<0.001). Even for the post-thaw gradient prepared sperm samples, a significant difference (p=0,01) was noted between the two groups.In conclusion, sperm preparation through gradient centrifugation prior to freezing improves the net yield in terms of progressive motility as opposed post-thaw preparation.

Place, publisher, year, edition, pages
2017.
Keywords [en]
Cryoinjury, slow freezing, cryoprotectants, sperm viability, vitrification
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349183OAI: oai:DiVA.org:uu-349183DiVA, id: diva2:1199897
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-23 Created: 2018-04-23 Last updated: 2018-04-23Bibliographically approved

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