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Sequencing of Cryptic loci regulator protein 1 (Clr1) gene inSchizosaccharomyces pombe strains shows the importanceof zinc-finger domains
2014 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Fission yeast Schizosaccharomyces pombe is a model organism for heterochromatic studiessince its heterochromatic mechanisms have similarities with higher eukaryotic organisms.Cryptic loci regulator protein 1 (Clr1) is somehow involved in epigenetic regulation in S.pombe. The protein has two known zinc-finger domains, but the proteinĀ“s function and theimportance of these domains are unknown. To identify possible important domains andprovide opportunities for further studies of Clr1 and its interactants, the nucleotide sequenceof Clr1 in twenty-two strains of S. pombe, with probable defect Clr1 protein, was determined.DNA was extracted from enriched strains and the gene amplified by PCR. The PCR productswere then purified and sequenced. The sequencing data revealed a point mutation in twentyonestrains and a duplication in one strain. The mutations found upstream the zinc-fingerdomains were nonsense mutations, all giving truncated protein products. The missensemutations inside the zinc-finger domains substituted the amino acids responsible forstabilizing the zinc ion. These results shows that the most important domains of Clr1 are thezinc-finger domains and that substitution of a zinc-binding amino acid probably can generatea defect protein. To fully understand the function of the zinc-finger domains and the functionof the rest of the protein further studies are needed.

Place, publisher, year, edition, pages
2014.
Keywords [en]
Heterochromatin, point mutation, epigenetics, Cys2His2, tandem CysTrpCysHis2
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349260OAI: oai:DiVA.org:uu-349260DiVA, id: diva2:1201015
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-24 Created: 2018-04-24 Last updated: 2018-04-24Bibliographically approved

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