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Evaluation of a new Particle EnhancedTurbidimetric Immunoassay with IgY antibodies fordetection of Calprotectin
2014 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Background: Calprotectin is released by neutrophils during inflammation. It is easily measured in faeces and therefore commonly used as a marker for inflammatory bowel disease (IBD).The gold standard method for analyzing calprotectin has for a long time been ELISA. Receiving results from ELISA takes days and is therefore not very cost efficient.Objective: The objective of this study was to evaluate a new, faster particle enhanced turbidimetric immunoassay (PETIA) for analyzing calprotectin using IgY-antibodies. Methods: Faeces samples (n=49), previously analyzed by using conventional ELISA, were analyzed on Mindray BS-380 in Uppsala, Sweden with the new PETIA. The study included how the clarity of the sample affected the results and also monitor calprotectins’ sustainability in faeces and plasma samples (n=3) over time and in different temperatures.Results: The results showed a total CV of 1.7 % for PETIA and linearity R²-values <0.75. The precision and reproducibility of the PETIA was good (R²=0.9972) but the correlation between methods were poor (R²=0.3305) even if samples <150 mg/kg calprotectin differed less. All faece samples <100 mg/kg calprotectin increased >100 % in seven days. The calprotectin concentrations in faeces were not affected by repeatedly freeze/thawing and the PETIA was not affected by the clarity of the sample.Conclusion: Calprotectin is stable for more than a week in plasma samples but for no more than 24h in faeces extracts. Calprotectin is not affected by repeatedly freeze/thawing. The PETIA is a good stabile method for analyzing calprotectin in faeces but may not be completely comparable to the ELISA

Place, publisher, year, edition, pages
2014.
Keywords [en]
PETIA, chicken antibody, stability, inflammatory bowel disease, irritable bowel syndrome.
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349261OAI: oai:DiVA.org:uu-349261DiVA, id: diva2:1201034
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-24 Created: 2018-04-24 Last updated: 2018-04-24Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
  • ieee
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Language
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Output format
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