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Comparison of two RIDA®GENE Parasitic Stool Panel multiplex real-time PCR kits for quantitative detection of four intestinal parasites.
2014 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Parasites cause symptoms like severe diarrhoea and abdominal pain and have long been a health issue in several countries. Giardia intestinalis, Entamoeba histolytica, Cryptosporidium spp, and Dientamoeba fragilis, the most common parasites analysed in laboratories, are usually detected using microscopy. A few laboratories have now embraced PCR as the main method for detection of these parasites.The aim of this study was to compare two different RIDA®GENE Parasitic Stool Panel kits, one which D. fragilis is analysed using a melting curve analysis and the other using a fluorescent hydrolysis probe.Using the old RIDA®GENE Parasitic Stool Panel as gold standard, the sensitivity, specificity, PPV and NPV for the new kit were 84.6 %, 100%, 100% and 95.6 % for G. intestinalis, 100%, 100%, 100% and 100% for Cryptosporidium spp. and 97.4 %, 100%, 94.6 % and 100% for D. fragilis. Unfortunately, no samples positive for E. histolytica by real-time PCR nor microscopy appeared in the laboratory during the period of evaluation of these two kits.Spectral overlapping between D. fragilis and G. intestinalis was quite common in this study, as was low signals for D. fragilis and the ICD (Internal control DNA) in each sample. Even though this was a problem, it did not interfere with the reliability of the test.The results were satisfactory and the new RIDA®GENE Parasitic Stool Panel kit is soon to be introduced as the kit used for detection of these four intestinal protozoa.

Place, publisher, year, edition, pages
2014.
Keywords [en]
Gastrointestinal infections, trofozoites, fluorescent hydrolysis probe, intercalated colour, melting curve
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-349264OAI: oai:DiVA.org:uu-349264DiVA, id: diva2:1201081
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Examiners
Available from: 2018-04-24 Created: 2018-04-24 Last updated: 2018-04-24Bibliographically approved

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