uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Unraveling the size-dependent optical properties of dissolved organic matter
Tech Univ Denmark, Natl Inst Aquat Resources, Sect Oceans & Arctic, Lyngby, Denmark..
Tech Univ Denmark, Natl Inst Aquat Resources, Sect Oceans & Arctic, Lyngby, Denmark..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Limnology.ORCID iD: 0000-0003-3509-8266
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Limnology. Univ Quebec Trois Rivieres, Res Ctr Watershed Aquat Ecosyst Interact RIVE, Trois Rivieres, PQ, Canada..
2018 (English)In: Limnology and Oceanography, ISSN 0024-3590, E-ISSN 1939-5590, Vol. 63, no 2, p. 588-601Article in journal (Refereed) Published
Abstract [en]

The size-dependent optical properties of dissolved organic matter (DOM) from four Swedish lakes were investigated using High Performance Size Exclusion Chromatography (HPSEC) in conjunction with online characterization of absorbance (240-600 nm) and fluorescence (excitation: 275 nm, emission: 300-600 nm). The molecular size of chromophoric DOM (CDOM) was consistently higher than that of fluorescent DOM (FDOM), with an average difference of 0.37 kDa. The relative abundance of FDOM vs. CDOM ranged from 0.3 to 0.7 across lakes, and increased with decreasing average molecular size. Across sites, the CDOM spectral slopes of the large molecular size fraction were highly similar while the low molecular size fraction differed and contributed to different bulk spectral slopes. Our results indicate structural congruence of high molecular size DOM across systems while lake trophic status determined the characteristics of the low size range. Furthermore, the combination of HPSEC and parallel factor analysis (HPSEC-PARAFAC2) allowed the decomposition of DOM fluorescence chromatograms. Three humic-like components and one protein-like component with broadly overlapping molecular size distributions were identified. This overlap provides further evidence for the supramolecular assembly hypothesis since fluorophores, as revealed by PARAFAC2, occur in aggregates of overlapping molecular size. Our results further suggest a link between the molecular size of these fluorophores and the associated supramolecular assemblies. This study demonstrates the potential for HPSEC and novel mathematical approaches to provide unprecedented insights into the relationship between optical and chemical properties of DOM in aquatic systems.

Place, publisher, year, edition, pages
WILEY , 2018. Vol. 63, no 2, p. 588-601
National Category
Environmental Sciences
Identifiers
URN: urn:nbn:se:uu:diva-350886DOI: 10.1002/lno.10651ISI: 000427104700006OAI: oai:DiVA.org:uu-350886DiVA, id: diva2:1209032
Funder
Swedish Research CouncilSwedish Research Council FormasKnut and Alice Wallenberg FoundationAvailable from: 2018-05-21 Created: 2018-05-21 Last updated: 2018-05-21Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full text

Authority records BETA

Tranvik, Lars

Search in DiVA

By author/editor
Tranvik, Lars
By organisation
Limnology
In the same journal
Limnology and Oceanography
Environmental Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 47 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf