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Host cell response upon exposure to Giardia intestinalis glycoproteins in vitro
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2018 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

Giardia intestinalis is an intestinal parasite responsible for a burden of millions of infections worldwide yearly, especially in children. Although the disease affects millions there is poor understanding of the pathophysiology of the parasite and how it causes disease.

Previous reports indicate highly transcribed inflammatory genes in response to the exposure to the parasite and to parasite released products. Zfp36 i.e. Tristetraprolin was also reported to be upregulated. This project aimed for identifying which molecules could induce or modulate the inflammation caused by the parasite on the host.

In this work we used differential gene expression analyses to identify the response of intestinal epithelial cells (IECs) to glycoproteins isolated from Giardia secreted products in vitro. The amount of glycoproteins added to IECs and inhibition of cysteine proteases affected the transcriptional response of inflammatory genes. There was a modulation on the Toll-Like receptor pathway which can affect the transcription of inflammatory genes and relate to the results obtained at RNA level.

This study provided a new insight into how Giardia’s secreted products (glycoproteins) interact with the host during giardiasis and shed light over on how some proteins interact with cells during the course of the disease.

Place, publisher, year, edition, pages
2018. , p. 35
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Identifiers
URN: urn:nbn:se:uu:diva-353938OAI: oai:DiVA.org:uu-353938DiVA, id: diva2:1219851
Educational program
Master Programme in Infection Biology
Supervisors
Examiners
Available from: 2018-06-19 Created: 2018-06-18 Last updated: 2018-06-19Bibliographically approved

Open Access in DiVA

The full text will be freely available from 2019-04-01 09:18
Available from 2019-04-01 09:18

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Department of Medical Biochemistry and Microbiology
Public Health, Global Health, Social Medicine and Epidemiology

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