uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Photosynthetic production of raw materials for jet fuel using cyanobacteria: In vivo study of recombinant oleate hydratase for isobutene production
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2018 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Aviation accounts for a significant part of the world’s CO2 emission from the transport sector, but sustainable jet fuels are not as developed or as commonly used as renewable fuels for road vehicles. Biofuels produced through photosynthesis in cyanobacteria are of interest to help solve the pressing issue of CO2 emissions and increased global warming. Isobutene, a volatile gas converted from isobutanol, could be assembled into sustainable jet fuel in a photo-biochemical system utilizing cyanobacteria. In this study, I examined the possibility of using metabolic engineering of Escherichia coli and the cyanobacterial strain Synechocystis PCC 6803 for enzymatic conversion of isobutanol to isobutene using recombinantly expressed oleate hydratase (OhyA), originating from four different organism.

Plasmids containing the kivd gene, coding for the enzyme α-ketoisovalerate decarboxylase (Kivd) which synthesise isobutanol, and the ohyA gene were constructed and inserted into E. coli and Synechocystis. The inserted OhyA variants were successfully expressed and showed isobutanol production in Synechocystis, confirming functionality of Kivd. From the experimental setup and OhyA variants used in this thesis, in vivo production of isobutene from isobutanol was not detectable in E. coli or Synechocystis. These results indicate OhyA inactivity for the conversion of isobutanol to isobutene in vivo using this particular experimental setup. For future in vitro studies, three OhyA variants were successfully purified by affinity chromatography. Two of these variants showed FAD binding. The findings of this study will contribute to ongoing research on characterization of OhyA and cyanobacterial conversion of CO2 to isobutene for jet fuel production. 

Place, publisher, year, edition, pages
2018. , p. 43
Series
UPTEC X ; 18 008
Keywords [en]
cyanobacteria, oleate hydratase, biofuel, protein expression, isobutene, isobutanol, synechocystis
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-354710OAI: oai:DiVA.org:uu-354710DiVA, id: diva2:1222375
Educational program
Molecular Biotechnology Engineering Programme
Supervisors
Examiners
Available from: 2018-06-21 Created: 2018-06-21 Last updated: 2018-06-21Bibliographically approved

Open Access in DiVA

The full text will be freely available from 2020-06-30 13:00
Available from 2020-06-30 13:00

By organisation
Biology Education Centre
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 34 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf