uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Prevalence of interfering antibodies in dogs and cats evaluated using a species-independent assay.
Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry.ORCID iD: 0000-0003-3161-0402
Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden..
Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden..
Show others and affiliations
2018 (English)In: Veterinary clinical pathology, ISSN 0275-6382, E-ISSN 1939-165X, Vol. 47, no 2, p. 205-212Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Interfering antibodies in human serum and plasma are known to react with mammalian antibodies in immunoassays and cause false-positive test results. Although this phenomenon was recently shown in companion animals, knowledge regarding immunoassay interference in veterinary medicine is very limited.

OBJECTIVES: The aims of this study were to set up a species-independent immunoassay procedure to detect interference in serum samples, to screen for interference in a cross-section of canine and feline patient samples from an animal hospital, and to determine if the detected interference could be neutralized using an immunoassay based on nonmammalian reagents.

METHODS: A 2-site sandwich-type interference assay was set up using commercially available mouse reagents. A total of 369 serum samples from 320 dogs and 263 samples from 218 cats were analyzed using the interference assay. Multiple samples were submitted from 36 dogs and 39 cats. Nineteen samples identified as interference-positive were analyzed in an assay using chicken antibodies.

RESULTS: Interference was detected in samples from 28 dogs (9%) and 10 cats (5%) screened with the interference assay. Except for 1 cat, consistent results were obtained for all 75 dogs and cats that submitted more than 1 sample. The interference was eliminated when analyzed in the chicken-based assay (P < .001).

CONCLUSIONS: Substances with reactivity toward mouse IgG can be detected in serum samples from dog and cat patients using a 2-site interference assay. The detected substances are most likely interfering antibodies, possibly originating from immunization with other mammalian species.

Place, publisher, year, edition, pages
2018. Vol. 47, no 2, p. 205-212
Keywords [en]
ELISA, heterophilic antibodies, immunoassay, interference, rheumatoid factors
National Category
Clinical Science
Identifiers
URN: urn:nbn:se:uu:diva-355212DOI: 10.1111/vcp.12612ISI: 000435286100011PubMedID: 29902338OAI: oai:DiVA.org:uu-355212DiVA, id: diva2:1227900
Available from: 2018-06-27 Created: 2018-06-27 Last updated: 2018-09-05Bibliographically approved

Open Access in DiVA

fulltext(637 kB)1 downloads
File information
File name FULLTEXT01.pdfFile size 637 kBChecksum SHA-512
56681423f93ff18a08d6ab33e660aa7b0dbb86289786f1d34254f25647c1ceb98fe806173a6c412b534cec0eb24ac2e0b508a217a1deaeb36624c3654d28febc
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Larsson, Anders

Search in DiVA

By author/editor
Larsson, Anders
By organisation
Clinical Chemistry
In the same journal
Veterinary clinical pathology
Clinical Science

Search outside of DiVA

GoogleGoogle Scholar
Total: 1 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 121 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf