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Studies on possible global suppressor mutations in the hisA gene of Salmonella enterica
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2018 (English)Report (Other academic)
Abstract [en]

Global suppressor mutations have recently been recognised as an important factor in enzyme evolution. These are mutations that compensate for deleterious mutations by stabilising the protein’s structure. Their implication in the development of resistance to antibiotics, including the ever-prevalent β-lactamase enzymes responsible for widespread resistance towards drugs such as ampicillin, has made global suppressors the focus of recent research. Model enzyme systems are often used to study how genes evolve in a laboratory system. In this project, the hisA gene of Salmonella enterica was studied; specifically, a set of previously-isolated mutations thought to represent global suppressors. Using an optimised method of λ Red Recombineering and techniques to measure the relative growth rate of bacteria, these experiments aimed to study possible compensatory mutations and understand how they affected fitness. In doing so we have found further mutations which appear to compensate for harmful mutations by reducing their fitness cost.

Place, publisher, year, edition, pages
2018. , p. 22
National Category
Evolutionary Biology Microbiology
Research subject
Biology with specialization in Molecular Evolution; Biology with specialization in Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-355306OAI: oai:DiVA.org:uu-355306DiVA, id: diva2:1228432
Note

Projektet utfördes som forskarpraktik på IMBIM, kurskod 3BL51 (15hp). Examinator var Dorothe Spillmann, IMBIM

Available from: 2018-06-28 Created: 2018-06-28 Last updated: 2018-06-28Bibliographically approved

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