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Debridement of Bacterial Biofilms with TiO2/H2O2 Solutions and Visible Light Irradiation
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.ORCID iD: 0000-0002-5496-9664
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.ORCID iD: 0000-0001-9529-650X
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials. (Nanoteknologi och funktionella material)ORCID iD: 0000-0003-4543-1130
2018 (English)In: International Journal of Biomaterials, ISSN 1687-8787, E-ISSN 1687-8795, Vol. 2018, article id 5361632Article in journal (Refereed) Published
Abstract [en]

Objectives. The aim of the study was to explore the debridement efficacy of different solutions of H2O2 and rutile particles against Staphylococcus epidermidis and Pseudomonas aeruginosa biofilms attached to titanium surfaces when exposed to visible light. Materials and Methods. Titanium discs cultivated with biofilms of Staphylococcus epidermidis or Pseudomonas aeruginosa were subjected for 1 min to suspensions consisting of rutile particles mixed with high (950 mM) or low (2 mM) concentrations of H2O2 under visible light irradiation (405 nm; 2.1 mW/cm2). Discs were rinsed and the degree of debridement was determined through scanning electron microscopy and viability assessment of the remaining bacteria using luminescence measurements and/or a metabolic activity assay. Results. Cleaning mixtures containing the higher concentration of H2O2 showed a significantly improved debridement compared to the negative control in all experiments. The addition of rutile particles was shown to have a statistically significant effect in one test with S. epidermidis. Limited evidence of the catalytic effect of visible light irradiation was seen, but effects were relatively small and statistically insignificant. Conclusions. H2O2 at a concentration of 950 mM proved to be the strongest contribution to the debridement and bactericidal effect of the cleaning techniques tested in this study.

Place, publisher, year, edition, pages
Hindawi Publishing Corporation, 2018. Vol. 2018, article id 5361632
National Category
Nano Technology Biomaterials Science
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials; Engineering Science with specialization in Materials Science
Identifiers
URN: urn:nbn:se:uu:diva-355674DOI: 10.1155/2018/5361632ISI: 000438846900001PubMedID: 30057613OAI: oai:DiVA.org:uu-355674DiVA, id: diva2:1230311
Funder
VINNOVA, E!8320Available from: 2018-07-03 Created: 2018-07-03 Last updated: 2018-09-26Bibliographically approved
In thesis
1. Bioactive Coatings and Antibacterial Approaches for Titanium Medical Implants
Open this publication in new window or tab >>Bioactive Coatings and Antibacterial Approaches for Titanium Medical Implants
2018 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The aim of this thesis was to characterize and manufacture coatings and surfaces with antibacterial properties and retained or enhanced bioactivity and biocompatibility. The aim was also to study the optimal composition and parameters of mixtures for debridement of bacterial biofilms on titanium surfaces. The mixtures contained TiO2 particles and H2O2 and were irradiated by light to activate reactive oxygen species (ROS) formation.

In the first part of the thesis, characterization of a thin, multifunctional hydroxyapatite (HA) coating was performed. The coating was applied to anodized cancellous bone screws with the purpose of stimulating local bone formation without bonding too firmly and providing local antibacterial effect. Specifications of the coating included a thickness of around 1 µm, high crystallinity, Ca/P ratio close to the theoretical value of 1.67 and comprise the functional groups of HA. Additionally, the adhesion of the coating to the substrate should be stronger than the cohesion of the coating. Characterization results showed that the coating met the specification for all criteria.        

In the second part of the thesis, titanium discs were soaked in H2O2 and subsequently in NaOH and Ca(OH)2 to acquire an antibacterial surface that at the same time is bioactive and biocompatible. The surface demonstrated bioactive properties, assessed by soaking in phosphate buffered saline for seven days in 37°C and examined in scanning electron microscopy and X-ray diffraction.

The third part of the thesis consisted of studying the ROS generation of TiO2/H2O2 mixtures irradiated with UV-Vis light, and to study the antibacterial effect of these mixtures on S. epidermidis Xen 43 and Pseudomonas aeruginosa biofilms on titanium surfaces. The generation of ROS from different TiO2 crystalline forms and different H2O2 concentrations under light UV-Vis irradiation was determined by rhodamine B degradation. It showed that rutile and 1-3.5 mM H2O2 resulted in the highest degradation of all combinations with almost 100% degradation under 365 nm light and 77% degradation under 405 nm light after 10 min.

The debridement of the S. epidermidis and P. aeruginosa biofilm discs showed that 0.95 M (3%) H2O2 was the most effective parameter for disinfection of the discs. The addition of TiO2 particles showed a significant extra effect in one of the three studies.

Place, publisher, year, edition, pages
Uppsala: Uppsala University, 2018. p. 63
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1712
Keywords
Bioactivity, antibacterial, titanium, hydrogen peroxide, hydroxyapatite, biomaterial
National Category
Other Materials Engineering
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-358253 (URN)978-91-513-0424-3 (ISBN)
Public defence
2018-10-12, Häggsalen, Ångströmlaboratoriet, Lägerhyddsvägen 1, Uppsala, 09:15 (English)
Opponent
Supervisors
Available from: 2018-09-20 Created: 2018-08-27 Last updated: 2018-10-15

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Janson, OscarEngqvist, HåkanWelch, Ken

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