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PREVALENCE OF HPV IN ANAL CARCINOMA AS REVEALED BY P16IMMUNOHISTOCHEMISTRY – A RETROSPECTIVE STUDY
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
2018 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Background

Squamous cell carcinoma of the anus is often considered related to chronic infection of

oncogene HPV types, primarily HPV16 and HPV18. P16 is a tumor suppressing protein

involved in the cell cycle. This protein is normally inhibited by the pRB (retinoblastoma)

protein, however HPV produces the protein E7 which binds to pRB and inactivates it.

Overexpression of p16 therefore indicates HPV-infection.

Purpose

The purpose of this study was to determine the likelihood of HPV-infection being the cause of

squamous cell carcinoma of the anus.

Materials and methods

The method used was immunohistochemistry using antibody towards p16-antigen. A

pathologist evaluated the tissues and graded them depending on the percentage of cancer cells

showing presence of p16. Tissues from 63 patients, 49 women, 14 men, were used.

Results

The results showed that 81 % of patients tested positive for p16. Cases showed positive

results in 85.7 % for women versus 64.3 % for men. Mann-Whitney U-test showed a

difference between genders with a two-tailed p value of 0.00386. Spearman’s rho test showed

no statistical monotone correlation between degree of overexpression of p16 and patient age.

Conclusion

In conclusion this study has shown that HPV-infection in 81 % of cases was the cause of

squamous cell carcinoma of the anus. The study further showed higher likelihood of

HPV-infection being the cause of the disease among female patients. The study could not

show patient age being a factor in likelihood of HPV-infection being the cause.

Place, publisher, year, edition, pages
2018. , p. 16
Keywords [en]
p16-expression, human papilloma virus, squamous cell carcinoma, IHC, biobank
National Category
Other Basic Medicine
Identifiers
URN: urn:nbn:se:uu:diva-356172OAI: oai:DiVA.org:uu-356172DiVA, id: diva2:1233642
External cooperation
Gävle sjukhus
Educational program
Biomedical Laboratory Science Programme
Supervisors
Examiners
Available from: 2018-11-26 Created: 2018-07-18 Last updated: 2018-11-26Bibliographically approved

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