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The effect of temperature on Pacifastacus leniusculus immunity
(English)In: Article in journal (Refereed) In press
Abstract [en]

Global climate change is an upcoming threat to marine as well as freshwater invertebrates and

the temperature around the globe is expected to rise with up to 4°C in the next decade. The

water temperature is known to affect many aspects of aquatic life including immune

responses and susceptibility to diseases. In this context, we studied the effect of temperature

on the defense system of the freshwater crayfish Pacifastacus leniusculus. Animals were

challenged with two pathogenic Gram-negative bacteria Aeromonas hydrophila and

Pseudomonas gessardii, as well as the bacterial cell wall component lipopolysaccharide

(LPS) at two different temperatures, one cold and one room temperature. The immune

responses were compared by means of differences in mortality, phagocytosis, bacterial

clearance, and the melanization reaction of the hemolymph at these two temperatures. We

observed that crayfish survived infections better at cold temperatures. The mortality rate was

zero at 6°C following A. hydrophila or LPS injections. Furthermore, the bacteria were

completely cleared from crayfish after they had been kept at this low temperature for more

than 9 days. We also observed a strong melanization reaction of hemolymph at 22°C when

stimulated with LPS, as well as with bacteria. Taken together, our results suggest that the

cellular immunity is more effective at low temperature in this cold-adapted animal and

pathogens are efficiently removed from the body by means of phagocytosis.

Keywords [en]
Aeromonas hydrophila, Pseudomonas gessardii, temperature, innate immunity, melanization, invertebrate
National Category
Immunology
Identifiers
URN: urn:nbn:se:uu:diva-356487OAI: oai:DiVA.org:uu-356487DiVA, id: diva2:1236117
Available from: 2018-07-31 Created: 2018-07-31 Last updated: 2018-07-31

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