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Overexpression of heparanase in mice promoted megakaryopoiesis
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Uppsala University, Science for Life Laboratory, SciLifeLab. Beijing Inst Transfus Med, Dept Tissue Engn, 27 Taiping Rd, Beijing 100850, Peoples R China.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Uppsala University, Science for Life Laboratory, SciLifeLab. Jiangxi Normal Univ, Coll Life Sci, 99 Ziyang Rd, Nanchang 330022, Jiangxi, Peoples R China.
Beijing Inst Transfus Med, Dept Tissue Engn, 27 Taiping Rd, Beijing 100850, Peoples R China.
Beijing Inst Transfus Med, Dept Tissue Engn, 27 Taiping Rd, Beijing 100850, Peoples R China.
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2018 (English)In: Glycobiology, ISSN 0959-6658, E-ISSN 1460-2423, Vol. 28, no 5, p. 269-275Article in journal (Refereed) Published
Abstract [en]

Heparanase, an endo-glucuronidase that specifically cleaves heparan sulfate (HS), is upregulated in several pathological conditions. In this study, we aimed to find a correlation of heparanase expression and platelets production. In the transgenic mice overexpressing human heparanase (Hpa-tg), hematological analysis of blood samples revealed a significantly higher number of platelets in comparison with wild-type (Ctr) mice, while no significant difference was found in leukocytes and red blood cell number between the two groups. Total number of thiazole orange positive platelets was increased in Hpa-tg vs. Ctr blood, reflecting a higher rate of platelets production. Concomitantly, megakaryocytes from Hpa-tg mice produced more and shorter HS fragments that were shed into the medium. Further, thrombopoietin (TPO) level was elevated in the liver and plasma of Hpa-tg mice. Together, the data indicate that heparanase expression promoted megakaryopoiesis, which may be through upregulated expression of TPO and direct effect of released HS fragments expressed in the megakaryocytes.

Place, publisher, year, edition, pages
OXFORD UNIV PRESS INC , 2018. Vol. 28, no 5, p. 269-275
Keywords [en]
heparan sulfate, heparanase, megakaryopoiesis, platelets
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:uu:diva-352573DOI: 10.1093/glycob/cwy011ISI: 000429486500003OAI: oai:DiVA.org:uu-352573DiVA, id: diva2:1237119
Funder
Swedish Research Council, 2015-02595Available from: 2018-08-07 Created: 2018-08-07 Last updated: 2018-08-07Bibliographically approved

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Tan, Ying-XiaZhang, XiaoLi, Jin-Ping

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Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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