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Impact of surfactants on the target recognition of Fab-conjugated PLGA nanoparticles
Univ Porto, Inst Invest & Inovacao Saude, Porto, Portugal;Univ Porto, INEB Inst Engn Biomed, Porto, Portugal;Univ Porto, IPATIMUP Inst Patol & Imunol Mol, Porto, Portugal;Univ Porto, ICBAS, Porto, Portugal.
Univ Porto, Inst Invest & Inovacao Saude, Porto, Portugal;Univ Porto, INEB Inst Engn Biomed, Porto, Portugal.
Univ Porto, Inst Invest & Inovacao Saude, Porto, Portugal;Univ Porto, IPATIMUP Inst Patol & Imunol Mol, Porto, Portugal.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Medical Radiation Science.
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2018 (English)In: European journal of pharmaceutics and biopharmaceutics, ISSN 0939-6411, E-ISSN 1873-3441, Vol. 127, p. 366-370Article in journal (Refereed) Published
Abstract [en]

Targeted drug delivery with nanoparticles (NPs) requires proper surface ligand presentation and availability. Surfactants are often used as stabilizers in the production of targeted NPs. Here, we evaluated the impact of surfactants on ligand functionalization and downstream molecular recognition. Our model system consisted of fluorescent poly(lactic-co-glycolic acid) (PLGA) NPs that were nanoprecipitated in one of a small panel of commonly-used surfactants followed by equivalent washes and conjugation of an engineered Fab antibody fragment. Size, polydispersity index and zeta potential were determined by dynamic light scattering and laser Doppler anemometry, and Fab presence on the NPs was assessed by enzyme-linked immunosorbent assay. Most importantly, Fab-decorated NP binding to the cell surface receptor was monitored by fluorescence-activated cell sorting. 2% polyvinyl alcohol, 1% sodium cholate, 0.5% Pluronic F127 (F127) and 2% Tween-80 were initially tested. Of the four surfactants tested, PLGA NPs in 0.5% F127 and 2% Tween-80 had the highest cell binding. These two surfactants were then retested in two different concentrations, 0.5% and 2%. The Fab-decorated PLGA NPs in 2% F127 had the highest cell binding. This study highlights the impact of common surfactants and their concentrations on the downstream targeting of ligand-decorated NPs. Similar principles should be applied in the development of future targeted nanosystems where surfactants are employed.

Place, publisher, year, edition, pages
Elsevier, 2018. Vol. 127, p. 366-370
Keywords [en]
Targeted nanoparticles, PLGA nanoparticles, Surfactant, Fab antibody fragment
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-357382DOI: 10.1016/j.ejpb.2018.03.005ISI: 000433650400039PubMedID: 29549023OAI: oai:DiVA.org:uu-357382DiVA, id: diva2:1241675
Funder
EU, Horizon 2020, NORTE-01-0145-FEDER-000012EU, Horizon 2020Available from: 2018-08-24 Created: 2018-08-24 Last updated: 2018-08-31Bibliographically approved

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Nestor, Marika

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