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Comparing the mechanical influence of vinculin, focal adhesion kinase and p53 in mouse embryonic fibroblasts.
2009 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 379, no 3, p. 799-801Article in journal (Refereed) Published
Abstract [en]

Cytoskeletal reorganization is an ongoing process when cells adhere, move or invade extracellular substrates. The cellular force generation and transmission are determined by the intactness of the actomyosin-(focal adhesion complex)-integrin connection. We investigated the intracellular course of action in mouse embryonic fibroblasts deficient in the focal adhesion proteins vinculin and focal adhesion kinase (FAK) and the nuclear matrix protein p53 using magnetic tweezer and nanoparticle tracking techniques. Results show that the lack of these proteins decrease cellular stiffness and affect cell rheological behavior. The decrease in cellular binding strength was higher in FAK- to vinculin-deficient cells, whilst p53-deficient cells showed no effect compared to wildtype cells. The intracellular cytoskeletal activity was lowest in wildtype cells, but increased in the following order when cells lacked FAK+p53>p53>vinculin. In summary, cell mechanical processes are differently affected by the focal adhesion proteins vinculin and FAK than by the nuclear matrix protein, p53.

Place, publisher, year, edition, pages
2009. Vol. 379, no 3, p. 799-801
National Category
Biophysics
Identifiers
URN: urn:nbn:se:uu:diva-358310DOI: 10.1016/j.bbrc.2008.12.124PubMedID: 19126403OAI: oai:DiVA.org:uu-358310DiVA, id: diva2:1242123
Available from: 2018-08-27 Created: 2018-08-27 Last updated: 2018-08-27

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Klemm, Anna H
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