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Evaluation of the precision ID whole MtDNA genome panel for forensic analyses
Med Univ Innsbruck, Inst Legal Med, Mullerstr 44, A-6020 Innsbruck, Austria.
Med Univ Innsbruck, Inst Legal Med, Mullerstr 44, A-6020 Innsbruck, Austria.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Medicinsk genetik och genomik.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Medicinsk genetik och genomik.
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2018 (English)In: Forensic Science International: Genetics, ISSN 1872-4973, E-ISSN 1878-0326, Vol. 35, p. 21-25Article in journal (Refereed) Published
Abstract [en]

Mitochondrial DNA (mtDNA) amplification and Massively Parallel Sequencing (MPS) using an early access version of the Precision ID Whole MtDNA Genome Panel (Thermo Fisher Scientific) and the Ion Personal Genome Machine (PGM) were evaluated using 15 forensically relevant samples. Samples were selected to represent typical forensic specimens for mtDNA analysis including hairs, hair shafts, swabs and ancient solid tissue samples (bones and teeth) that were stored in the freezer for up to several years after having been typed with conventional Sanger-type Sequencing and Capillary Electrophoresis. The MPS haplotypes confirmed the earlier results in all samples and provided additional sequence information that improved discrimination power and haplogroup estimation. The results raised the appetite for further experiments to validate and apply the new technology in forensic practice.

Place, publisher, year, edition, pages
2018. Vol. 35, p. 21-25
Keywords [en]
Mitochondrial DNA, Mitogenome, Massively parallel sequencing, Next generation sequencing, Degraded DNA, Mini-amplicon, Primer tiling, Forensic genetics
National Category
Forensic Science Medical Genetics
Identifiers
URN: urn:nbn:se:uu:diva-358367DOI: 10.1016/j.fsigen.2018.03.013ISI: 000434806300008PubMedID: 29626805OAI: oai:DiVA.org:uu-358367DiVA, id: diva2:1243409
Available from: 2018-08-31 Created: 2018-08-31 Last updated: 2018-08-31Bibliographically approved

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Bus, Magdalena M.Allen, Marie

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