uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Transfection of crayfish hematopoietic tissue cells
State Ocean Adm, State Key Lab Breeding Base Marine Genet Resource, Key Lab Marine Genet Resources,Inst Oceanog 3, Fujian Key Lab Marine Genet Resources,South China, Xiamen 361005, Peoples R China.
State Ocean Adm, State Key Lab Breeding Base Marine Genet Resource, Key Lab Marine Genet Resources,Inst Oceanog 3, Fujian Key Lab Marine Genet Resources,South China, Xiamen 361005, Peoples R China.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology.
Show others and affiliations
2018 (English)In: Developmental and Comparative Immunology, ISSN 0145-305X, E-ISSN 1879-0089, Vol. 88, p. 70-76Article in journal (Refereed) Published
Abstract [en]

Transfection is a powerful tool useful for studying gene function. Establishing transfection methods that enable highly efficient DNA uptake has become increasingly important. The crayfish hematopoietic tissue (Hpt) cell cultures have been proven to be suitable for studies on immunity and cell differentiation in crustaceans including shrimps, but no efficient gene transfer and expression method is available for these cells. Here we report a novel and highly efficient DNA transfection system based on electroporation. This method depends on a recombinant plasmid with the promoter from white spot syndrome virus immediate-early gene wsv249. This plasmid could be introduced into primary cells and efficiently express foreign genes by electroporation. By optimizing different electroporation parameters, more than 30% transfection efficiency could be achieved with the relative viability of cells around 50%. This is the first report of gene introduction to crayfish Hpt cells and will be useful for the expanding our research on crustacean immunity.

Place, publisher, year, edition, pages
2018. Vol. 88, p. 70-76
Keywords [en]
Transfection, Electroporation, EGFP reporter gene expression, WSSV IE gene promoter, Crayfish hematopoietic tissue cells
National Category
Immunology
Identifiers
URN: urn:nbn:se:uu:diva-364121DOI: 10.1016/j.dci.2018.07.005ISI: 000443668600009PubMedID: 30003890OAI: oai:DiVA.org:uu-364121DiVA, id: diva2:1259276
Funder
Swedish Research Council, VR 621-2012-2418Swedish Research Council Formas, 2011-606Available from: 2018-10-29 Created: 2018-10-29 Last updated: 2018-10-29Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Authority records BETA

Söderhäll, IreneSöderhäll, Kenneth

Search in DiVA

By author/editor
Söderhäll, IreneSöderhäll, Kenneth
By organisation
Comparative Physiology
In the same journal
Developmental and Comparative Immunology
Immunology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 27 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf