uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Effect of bilayer charge on lipoprotein lipid exchange
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy. Univ Copenhagen, Dept Pharm, Univ Pk 2, DK-2100 Copenhagen, Denmark.
Malmo Univ, Res Ctr Biointerfaces, Dept Biomed Sci & Biofilms, Malmo, Sweden.
Malmo Univ, Res Ctr Biointerfaces, Dept Biomed Sci & Biofilms, Malmo, Sweden.ORCID iD: 0000-0001-6547-5427
Inst Laue Langevin, Grenoble, France;Univ Kent, Sch Phys Sci, Canterbury, Kent, England.
Show others and affiliations
2018 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 168, p. 117-125Article in journal (Refereed) Published
Abstract [en]

Lipoproteins play a key role in the onset and development of atherosclerosis, the formation of lipid plaques at blood vessel walls. The plaque formation, as well as subsequent calcification, involves not only endothelial cells but also connective tissue, and is closely related to a wide range of cardiovascular syndromes, that together constitute the number one cause of death in the Western World. High (HDL) and low (LDL) density lipoproteins are of particular interest in relation to atherosclerosis, due to their protective and harmful effects, respectively. In an effort to elucidate the molecular mechanisms underlying this, and to identify factors determining lipid deposition and exchange at lipid membranes, we here employ neutron reflection (NR) and quartz crystal microbalance with dissipation (QCM-D) to study the effect of membrane charge on lipoprotein deposition and lipid exchange. Dimyristoylphosphatidylcholine (DMPC) bilayers containing varying amounts of negatively charged dimyristoylphosphatidylserine (DMPS) were used to vary membrane charge. It was found that the amount of hydrogenous material deposited from either HDL or LDL to the bilayer depends only weakly on membrane charge density. In contrast, increasing membrane charge resulted in an increase in the amount of lipids removed from the supported lipid bilayer, an effect particularly pronounced for LDL. The latter effects are in line with previously reported observations on atherosclerotic plaque prone regions of long-term hyperlipidaemia and type 2 diabetic patients, and may also provide some molecular clues into the relation between oxidative stress and atherosclerosis. 

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2018. Vol. 168, p. 117-125
Keywords [en]
Atherosclerosis, HDL, LDL, Lipid exchange, Lipoprotein, Neutron reflection
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-364466DOI: 10.1016/j.colsurfb.2018.01.043ISI: 000443630200016PubMedID: 29422308OAI: oai:DiVA.org:uu-364466DiVA, id: diva2:1259915
Funder
Swedish Research Council, 2014-3981Swedish Research Council, 2016-05157Available from: 2018-10-31 Created: 2018-10-31 Last updated: 2018-10-31Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Authority records BETA

Browning, Kathryn L.Malmsten, M

Search in DiVA

By author/editor
Browning, Kathryn L.Maric, SelmaMalmsten, M
By organisation
Department of Pharmacy
In the same journal
Colloids and Surfaces B: Biointerfaces
Physical Chemistry

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 8 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf