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The endoplasmic reticulum-resident collagen chaperone Hsp47 interacts with and promotes the secretion of decorin, fibromodulin, and lumican
Oregon Hlth & Sci Univ, Dept Biochem & Mol Biol, Portland, OR 97239 USA;Shriners Hosp Children, Res Dept, Portland, OR 97239 USA.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Uppsala Univ, Dept Med Biochem & Microbiol, S-75237 Uppsala, Sweden.ORCID iD: 0000-0003-2789-6276
Oregon Hlth & Sci Univ, Dept Biochem & Mol Biol, Portland, OR 97239 USA;Shriners Hosp Children, Res Dept, Portland, OR 97239 USA.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.ORCID iD: 0000-0002-6600-9302
2018 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 293, no 35, p. 13707-13716Article in journal (Refereed) Published
Abstract [en]

The build-up of diversified and tissue-specific assemblies of extracellular matrix (ECM) proteins depends on secreted and cell surface-located molecular arrays that coordinate ECM proteins into discrete designs. The family of small leucine-rich proteins (SLRPs) associates with and dictates the structure of fibrillar collagens, which form the backbone of most ECM types. However, whether SLRPs form complexes with proteins other than collagens is unclear. Here, we demonstrate that heat shock protein 47 (Hsp47), a well-established endoplasmic reticulum-resident collagen chaperone, also binds the SLRPs decorin, lumican, and fibromodulin with affinities comparable with that in the Hsp47-type I collagen interaction. Furthermore, we show that a lack of Hsp47 inhibits the cellular secretion of decorin and lumican. Our results expand the understanding of the concerted molecular interactions that control the secretion and organization of a functional collagenous ECM.

Place, publisher, year, edition, pages
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC , 2018. Vol. 293, no 35, p. 13707-13716
Keywords [en]
endoplasmic reticulum (ER), collagen, small leucine-rich proteoglycan (SLRP), protein secretion, protein-protein interaction, chaperone, decorin, fibromodulin, heat shock protein (HSP), extracellular matrix, Hsp47, lumican, small leucine-rich protein
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-364385DOI: 10.1074/jbc.RA117.000758ISI: 000443375500029PubMedID: 30002123OAI: oai:DiVA.org:uu-364385DiVA, id: diva2:1261667
Available from: 2018-11-08 Created: 2018-11-08 Last updated: 2018-11-08Bibliographically approved

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Rubin, KristoferKalamajski, Sebastian

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