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Growth Media Filtration Using Nanocellulose-based Virus Removal Filter for Upstream Biopharmaceutical Processing
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials. (Nanotechnology and Functional Materials)
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials. (Nanotechnology and Functional Materials)
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials. (Nanoteknologi och funktionella material)
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials. (Nanotechnology and Functional Materials)ORCID iD: 0000-0002-8105-2317
2019 (English)In: Journal of Membrane Science, ISSN 0376-7388, E-ISSN 1873-3123, Vol. 572, p. 464-474Article in journal (Refereed) Published
Abstract [en]

The feasibility of using nanocellulose-based mille-feuille filter paper for upstream applications in serum-free growth media filtration, i.e. Dulbecco’s modified Eagle’s medium (DMEM) and Luria-Bertani medium (LBM), was tested. The filter performance with respect to F.174 bacteriophage (28nm) removal as a model small-size virus was characterized for filters of varying thicknesses, i.e. 11 and 33 mu m, at varying operating pressures, i.e. 1 and 3bar. The filters demonstrated generally good model small-size virus removal properties with LRV = 5, especially for 33 mu m filters. The 33 mu m filters were more robust and exhibited better virus removal and throughput properties than 11 mu m filters, although their flux was generally lower. The performance of the 33 and 11 mu m nanocellulose-based filter papers was further verified for upscaled bioporcessing by 10-fold increase in the loading volume. The results of the present work show that the 33 mu m nanocellulose-based filter paper could be an interesting alternative for large volume cell culture medium filtration during upstream bioprocessing.

Place, publisher, year, edition, pages
2019. Vol. 572, p. 464-474
Keywords [en]
Cell culture media, Phi Chi 174 bacteriophage, size-exclusion virus filtration, filter fouling, microorganism contamination
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
URN: urn:nbn:se:uu:diva-366416DOI: 10.1016/j.memsci.2018.11.002ISI: 000453402100046OAI: oai:DiVA.org:uu-366416DiVA, id: diva2:1264554
Funder
Knut and Alice Wallenberg Foundation, 2013.0190Stiftelsen Olle Engkvist Byggmästare, 2015/717Available from: 2018-11-20 Created: 2018-11-20 Last updated: 2020-01-29Bibliographically approved

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Manukyan, LevonGustafsson, SimonMihranyan, Albert

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